CD8 alpha ELISA Pair Set | CD8A elisa pair set

CD8 alpha Matched ELISA Antibody Pair Set, Mouse

Cat. Num. AAA8118495

• Synonyms: CD8 alpha; CD8 alpha Matched ELISA Antibody Pair Set; Mouse; Mouse CD8a ELISA Pair Set; CD8a molecule; BB154331 Matched ELISA Antibody Pair Set; Ly-2 Matched ELISA Antibody Pair Set; Ly-35 Matched ELISA Antibody Pair Set; Ly-B Matched ELISA Antibody Pair Set; Lyt-2 Matched ELISA Antibody Pair Set; CD8A elisa pair set

• Specificity: Quantitative determination of Mouse CD8 alpha

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 46.88-3000pg/mL
• Sensitivity: 46.9pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of rabbit anti-mouse CD8a monoclonal antibody. Dilute to a working concentration of 2ug/mL in CBS before coating. ; 2. Detection Antibody 0.4 mg/mL rabbit anti-mouse CD8a polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 40 ng of recombinant mouse CD8a. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3ng/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CD8A elisa pair set

T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B-and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

This CD8 alpha Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CD8 alpha. It contains Mouse CD8 alpha capture antibody, Mouse CD8 alpha detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse CD8 alpha protein.

References

References Devine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.