CD81 recombinant protein

CD81 Protein, Human, Recombinant

Cat. Num. AAA8121650

• Gene Names: CD81; S5.7; CVID6; TAPA1; TSPAN28
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CD81; CD81 Protein; Human; Recombinant; Human CD81/TAPA-1 Protein; CVID6 Protein; S5.7 Protein; TAPA1 Protein; TSPAN28 Protein; CD81 molecule; CD81 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Phe113-Lys201

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the human CD81 (NP_004347.1)(Phe113-Lys201) was expressed with two additional amino acids (Gly&Pro) at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD81 recombinant protein

Background: CD81, also known as TAPA-1, belongs to the transmembrane 4 superfamily, also known as the tetraspanin family. Members of this family mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.CD81 is a widely expressed cell-surface protein involved in an astonishing variety of biologic responses. It is related to adhesion, morphology, activation, proliferation, and differentiation of B, T, and other cells. On B cells CD81 is part of a complex with CD21, CD19, and Leu13. This complex reduces the threshold for B cell activation via the B cell receptor by bridging Ag specific recognition and CD21-mediated complement recognition.

References

Petracca R. et al., 2000, J Virol. 74 (10): 4824-30. Bartosch B. et al., 2003, The Journal of Biological Chemistry. 278 (43): 41624-30. Clark KL. et al., 2001, Journal of Immunology. 167 (9): 5115-21.

NCBI and Uniprot Product Information

• NCBI GI #: 4757944
• NCBI GeneID: 975
• NCBI Accession #: NP_004347.1
• NCBI GenBank Nucleotide #: NM_004356.3
• UniProt Accession #: P60033; P18582; Q5U0J6
• Molecular Weight: 25,809 Da
• NCBI Official Full Name: CD81 antigen isoform 1
• NCBI Official Synonym Full Names: CD81 molecule
• NCBI Official Symbol: CD81
• NCBI Official Synonym Symbols: S5.7; CVID6; TAPA1; TSPAN28
• NCBI Protein Information: CD81 antigen; tspan-28; tetraspanin-28; 26 kDa cell surface protein TAPA-1; CD81 antigen (target of antiproliferative antibody 1)
• UniProt Protein Name: CD81 antigen
• Protein Family: CD81 antigen
• UniProt Gene Name: CD81
• UniProt Synonym Gene Names: TAPA1; TSPAN28; Tspan-28

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell.