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Western Blot (WB) (Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: Human normal liver tissue Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 seconds)

Rabbit anti-Human HGD Polyclonal Antibody | anti-HGD antibody

HGD Antibody

Gene Names
HGD; AKU; HGO
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
HGD; Polyclonal Antibody; HGD Antibody; AKU; HGO; anti-HGD antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total HGD protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
445
Applicable Applications for anti-HGD antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:10-1:50
Immunogen
Fusion protein of human HGD
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: Human normal liver tissue Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 seconds)

Western Blot (WB) (Gel: 8%SDS-PAGE Lysate: 40 μ g Lane: Human normal liver tissue Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)
Related Product Information for anti-HGD antibody
This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.
Product Categories/Family for anti-HGD antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,964 Da
NCBI Official Full Name
homogentisate 1,2-dioxygenase
NCBI Official Synonym Full Names
homogentisate 1,2-dioxygenase
NCBI Official Symbol
HGD
NCBI Official Synonym Symbols
AKU; HGO
NCBI Protein Information
homogentisate 1,2-dioxygenase
UniProt Protein Name
Homogentisate 1,2-dioxygenase
UniProt Gene Name
HGD
UniProt Synonym Gene Names
HGO
UniProt Entry Name
HGD_HUMAN

Uniprot Description

HGD: Defects in HGD are the cause of alkaptonuria (AKU). AKU is an autosomal recessive error of metabolism characterized by an increase in the level of homogentisic acid. The clinical manifestations of AKU are urine that turns dark on standing and alkalinization, black ochronotic pigmentation of cartilage and collagenous tissues, and spine arthritis. Belongs to the homogentisate dioxygenase family.

Protein type: Amino Acid Metabolism - tyrosine; EC 1.13.11.5; Oxidoreductase

Chromosomal Location of Human Ortholog: 3q13.33

Cellular Component: cytosol

Molecular Function: homogentisate 1,2-dioxygenase activity; identical protein binding; protein binding

Biological Process: L-phenylalanine catabolic process

Disease: Alkaptonuria

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Product Notes

The HGD hgd (Catalog #AAA9416071) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HGD Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HGD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the HGD hgd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HGD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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