SDS-PAGE
PRMT5 / SKB1 recombinant protein
Recombinant Human PRMT5 / SKB1 Protein (His & DYKDDDDK tag)
Gene Names
CD7; GP40; TP41; Tp40; LEU-9
Purity
> 85 % as determined by SDS-PAGE
Synonyms
PRMT5 / SKB1; Recombinant Human PRMT5 / SKB1 Protein (His & DYKDDDDK tag); PRMT5; HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; PRMT5 / SKB1 recombinant protein
Host
Human Cells
Purity/Purification
> 85 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM EDTA, 0.5PMSF, 0.5mM, TCEP, 25% glycerol
Sequence Length
240
Application Notes
The recombinant human PRMT5 consists of 655 amino acids and predictes a molecular mass of 75 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhPRMT5 is approximately 65 kDa.
Predicted N Terminal
Met
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for PRMT5 / SKB1 recombinant protein
Background: Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation, signalling and subcellular localization of proteins and nucleic acids. Protein arginine methyltransferase 5 (PRMT5) is a member of the protein arginine N-methyltransferases (PRMT)family, and exists as at least homodimers and homotetramers, or homooligomers mediated by disulfide bonds and non-covalent association ubiquitously. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), and thus plays a role in the assembly and biogenesis of snRNP core particles. PRMT5 methylates histone H2A and H4 'Arg-3' during germ cell development, as well as histone H3 'Arg-8', which may repress transcription. PRMT5 also methylates SUPT5H and regulates its transcriptional elongation properties. Additionally, it is also suggested that PRMT5 negatively regulates cyclin E1 promoter activity and cellular proliferation.
Description: A DNA sequence encoding the human PRMT5 (NP_006128.1) (Ala 2-Leu 637) was expressed, fused with a polyhistidine tag at the C-terminus and a DYKDDDDK tag at the N-terminus.
Description: A DNA sequence encoding the human PRMT5 (NP_006128.1) (Ala 2-Leu 637) was expressed, fused with a polyhistidine tag at the C-terminus and a DYKDDDDK tag at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,409 Da
NCBI Official Full Name
T-cell antigen CD7
NCBI Official Synonym Full Names
CD7 molecule
NCBI Official Symbol
CD7
NCBI Official Synonym Symbols
GP40; TP41; Tp40; LEU-9
NCBI Protein Information
T-cell antigen CD7
UniProt Protein Name
T-cell antigen CD7
UniProt Gene Name
CD7
NCBI Description
This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. [provided by RefSeq, Jul 2008]
Uniprot Description
CD7: Not yet known.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: integral to membrane; membrane; plasma membrane
Molecular Function: receptor activity
Biological Process: immune response; T cell activation
Research Articles on PRMT5 / SKB1
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Product Notes
The PRMT5 / SKB1 cd7 (Catalog #AAA2545933) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human PRMT5 consists of 655 amino acids and predictes a molecular mass of 75 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhPRMT5 is approximately 65 kDa. Researchers should empirically determine the suitability of the PRMT5 / SKB1 cd7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRMT5 / SKB1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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