CD69 recombinant protein

Recombinant Human CD69 Protein (N-His)

Cat. Num. AAA2557303

• Gene Names: CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: CD69; Recombinant Human CD69 Protein (N-His); Early activation antigen CD69; Activation inducer molecule; AIM; BL-AC/P26; C-type lectin domain family 2 member C; EA1; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; CLEC2C; CD69 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: Gly64-Lys199
• Sequence Length: 199

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: N-His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for CD69 recombinant protein

Protein Construction: Recombinant Human CD69 is produced by our mammalian expression system and the target gene encoding Gly64-Lys199 is expressed with a 8His tag at the N-terminus.

Background: Human Early Activation Antigen CD69 (CD69) is a type 2 transmembrane glycoprotein in the C-type lectin family. It plays roles in immune cell trafficking, inflammation, T cell memory, and humoral immune responses. CD69 is expressed on the cell surface as an approximately 60 kDa disulfide-linked homodimer. It is found on CD4+ T cells, CD8+ T cells, NK cells, NKT cells, gamma delta cells dendritic cells (DC) and is up-regulated on activated T cells and DC. Ligation of CD69 on DC induces IL2 production, leading to T cell proliferation. CD69 is important for the homing of CD4+ T cells and plasmablasts to the bone marrow but inhibits the migration of dermal DC to draining lymph nodes. It supports the expression of multiple chemokines and chemokine receptors but suppresses the expression of others. It associates with and negatively regulates S1P1 expression on DC and CD4+ T cells, resulting in a decreased chemotactic response to S1P. The direct interaction of CD69 with Galectin-1 contributes to the ability of CD69 to limit Th17 mediated inflamamtion while supporting the differentiation of regulatory T cells.

NCBI and Uniprot Product Information

• NCBI GI #: 584906
• NCBI GeneID: 969
• UniProt Accession #: Q07108
• Molecular Weight: Molecular Mass: 16.9kDa; Actual Protein Molecular Mass: 18-28kDa
• NCBI Official Full Name: Early activation antigen CD69
• NCBI Official Synonym Full Names: CD69 molecule
• NCBI Official Symbol: CD69
• NCBI Official Synonym Symbols: AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
• NCBI Protein Information: early activation antigen CD69
• UniProt Protein Name: Early activation antigen CD69
• Protein Family: Early activation antigen
• UniProt Gene Name: CD69
• UniProt Synonym Gene Names: CLEC2C; AIM
• UniProt Entry Name: CD69_HUMAN

NCBI Description

This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]

Uniprot Description

Function: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

Subunit structure: Homodimer; disulfide-linked. Ref.6 Ref.7 Ref.8

Subcellular location: Membrane; Single-pass type II membrane protein.

Tissue specificity: Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.

Developmental stage: Earliest inducible cell surface glycoprotein acquired during lymphoid activation.

Induction: By antigens, mitogens or activators of PKC on the surface of T and B-lymphocytes. By interaction of IL-2 with the p75 IL-2R on the surface of NK cells.

Post-translational modification: Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

Sequence similarities: Contains 1 C-type lectin domain.