Rabbit anti-Human MeCP2 Polyclonal Antibody | anti-MECP2 antibody

MeCP2, NT (S80) (MECP2, Methyl-CpG-binding protein 2)

Cat. Num. AAA6001925

• Gene Names: MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: MeCP2; Polyclonal Antibody; NT (S80) (MECP2; Methyl-CpG-binding protein 2); Anti -MeCP2; anti-MECP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-MECP2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MeCP2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MeCP2 Antibody (pS80) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).)

Immunohistochemistry (IHC)

(MeCP2 Antibody (N-term S80) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MeCP2 Antibody (N-term S80) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MeCP2 Antibody (N-term S80) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MECP2 antibody

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq].

Product Categories/Family for anti-MECP2 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 160707950
• NCBI GeneID: 4204
• NCBI Accession #: NP_001104262.1
• NCBI GenBank Nucleotide #: NM_001110792.1
• UniProt Accession #: P51608; O15233; Q6QHH9; Q7Z384
• Molecular Weight: 52,441 Da
• NCBI Official Full Name: methyl-CpG-binding protein 2 isoform 2
• NCBI Official Synonym Full Names: methyl CpG binding protein 2 (Rett syndrome)
• NCBI Official Symbol: MECP2
• NCBI Official Synonym Symbols: RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
• NCBI Protein Information: methyl-CpG-binding protein 2; meCp-2 protein
• UniProt Protein Name: Methyl-CpG-binding protein 2
• Protein Family: Methyl-CpG-binding protein
• UniProt Gene Name: MECP2
• UniProt Synonym Gene Names: MeCp-2 protein; MeCp2
• UniProt Entry Name: MECP2_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]

Uniprot Description

MECP2: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Interacts with FNBP3. Interacts with CDKL5. Present in all adult somatic tissues tested. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: nucleoplasm; heterochromatin; extracellular space; cytosol; nucleus

Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; methyl-CpG binding; DNA binding; double-stranded methylated DNA binding; protein N-terminus binding; chromatin binding; transcription corepressor activity; transcription factor activity; transcription factor binding

Biological Process: catecholamine secretion; startle response; behavioral fear response; genetic imprinting; adult locomotory behavior; positive regulation of transcription, DNA-dependent; chromatin silencing; pathogenesis; neurological control of breathing; negative regulation of transcription from RNA polymerase II promoter; proprioception; sensory perception of pain; negative regulation of histone methylation; neuron maturation; post-embryonic development; phosphatidylcholine metabolic process; negative regulation of histone acetylation; protein localization; synaptogenesis; positive regulation of cell proliferation; dendrite development; visual learning; cerebellum development; histone acetylation; negative regulation of neuron apoptosis; mitochondrial electron transport, ubiquinol to cytochrome c; transcription, DNA-dependent; ventricular system development; glutamine metabolic process; respiratory gaseous exchange; social behavior; cardiolipin metabolic process; histone methylation; glucocorticoid metabolic process; positive regulation of synaptogenesis; regulation of systemic arterial blood pressure by neurological process; long-term memory; inositol metabolic process; response to hypoxia; negative regulation of transcription, DNA-dependent; regulation of excitatory postsynaptic membrane potential

Disease: Rett Syndrome; Mental Retardation, X-linked, Syndromic 13; Autism, Susceptibility To, X-linked 3; Lubs X-linked Mental Retardation Syndrome; Angelman Syndrome; Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations