Protectin (CD59) Recombinant Protein | CD59 recombinant protein

Recombinant Protectin (CD59)

Cat. Num. AAA2010498

• Gene Names: Cd59; Cd59a; Cd59b; MACIF; MACIP; MAC-IP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protectin (CD59); Recombinant Protectin (CD59); CD59 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-LRCYNCLD PVSSCKTNST CSPNLDACLV AVSGKQVYQQ CWRFSDCNAK FILSRLEIAN VQYRCCQADL CNKSFEDKPN NGAIS
• Sequence Length: 126

• Applicable Applications for CD59 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Leu23~Ser105 (Accession # P27274) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 6978635
• NCBI GeneID: 25407
• NCBI Accession #: NP_037057.1
• NCBI GenBank Nucleotide #: NM_012925.1
• UniProt Accession #: P27274
• Molecular Weight: 15.0kDa
• NCBI Official Full Name: CD59 glycoprotein
• NCBI Official Synonym Full Names: CD59 molecule, complement regulatory protein
• NCBI Official Symbol: Cd59
• NCBI Official Synonym Symbols: Cd59a; Cd59b; MACIF; MACIP; MAC-IP
• NCBI Protein Information: CD59 glycoprotein; protectin; CD59 antigen; Cb59b molecule; MAC-inhibitory protein; membrane attack complex inhibition factor; CD59b moleucle, complement regulatory protein
• UniProt Protein Name: CD59 glycoprotein
• Protein Family: CD59 glycoprotein
• UniProt Gene Name: Cd59
• UniProt Synonym Gene Names: MAC-IP; MACIF
• UniProt Entry Name: CD59_RAT

NCBI Description

may play a role in regulation of complement; may bind complement proteins C8 and C9 [RGD, Feb 2006]

Uniprot Description

CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Protein type: Membrane protein, GPI anchor

Cellular Component: anchored to external side of plasma membrane; compact myelin; extracellular space; focal adhesion; cell surface; plasma membrane; sarcolemma; vesicle

Molecular Function: complement binding

Biological Process: negative regulation of cytolysis; cell surface receptor linked signal transduction; negative regulation of complement activation; regulation of complement activation; negative regulation of activation of membrane attack complex; positive regulation of T cell proliferation; negative regulation of apoptosis