Lymphocyte Function Associated Antigen 3 Recombinant Protein | LFA3 recombinant protein

Recombinant Lymphocyte Function Associated Antigen 3 (LFA3)

Cat. Num. AAA2028904

• Gene Names: CD58; ag3; LFA3; LFA-3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Lymphocyte Function Associated Antigen 3; Recombinant Lymphocyte Function Associated Antigen 3 (LFA3); LFA3 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MVAGSDAGRA LGVLSVVCLL HCFGFISCFS QQIYGVVYGN VTFHVPSNVP LKEVLWKKQK DKVAELENSE FRAFSSFKNR VYLDTVSGSL TIYNLTSSDE DEYEMESPNI TDTMKFFLYV LESLPSPTLT CALTNGSIEV QCMIPEHYNS HRGLIMYSWD CPMEQCKRNS TSIYFKMENDLPQKIQCTLS NPLFNTTSSI ILTTCIPSSG HSRH
• Sequence Length: 248

• Applicable Applications for LFA3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 54.1kDa
• Accurate Molecular Mass (KD): 54kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ala25~Lys182
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 221316575
• NCBI GeneID: 965
• NCBI Accession #: NP_001138294.1
• NCBI GenBank Nucleotide #: NM_001144822.1
• Molecular Weight: 27,960 Da
• NCBI Official Full Name: lymphocyte function-associated antigen 3 isoform 2
• NCBI Official Synonym Full Names: CD58 molecule
• NCBI Official Symbol: CD58
• NCBI Official Synonym Symbols: ag3; LFA3; LFA-3
• NCBI Protein Information: lymphocyte function-associated antigen 3
• UniProt Protein Name: Lymphocyte function-associated antigen 3
• UniProt Gene Name: CD58
• UniProt Synonym Gene Names: LFA3; Ag3
• UniProt Entry Name: LFA3_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

CD58: Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen- presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: cell surface; membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; cell adhesion; blood coagulation; leukocyte migration