Mouse anti-Mouse FXYD Domain-containing Ion Transport Regulator 1 Monoclonal Antibody | anti-Fxyd1 antibody

FXYD Domain-containing Ion Transport Regulator 1, phosphorylated (Ser68) (Phospholemman, FLM, Fxyd1, Plm) (Azide free) (HRP)

Cat. Num. AAA6121502

• Gene Names: Fxyd1; Plm; Pml; 0610012C17Rik; 1110006M24Rik
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: FXYD Domain-containing Ion Transport Regulator 1; Monoclonal Antibody; phosphorylated (Ser68) (Phospholemman; FLM; Fxyd1; Plm) (Azide free) (HRP); anti-Fxyd1 antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10i44
• Specificity: Recognizes mouse PLM when phosphorylated at Ser68 at ~10kD.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 95

• Applicable Applications for anti-Fxyd1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic phosphopeptide mapping to a fragment of residues surrounding Ser68 human PLM, UniProt Accession #NP_062376; Q9Z239 Cellular Localization: Membrane; Single-pass Type 1 membrane protein
• Conjugate: HRP
• Cellular Localization: Membrane; Single-Pass Type 1 Membrane Protein
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS631758 in 293 cells transfected with a plasmid encoding PLM.)

Product Categories/Family for anti-Fxyd1 antibody

Antibodies; Ion Channel

References

1. Crowell, K.J., et al., Biochim. Biophys. Acta 1645(1):15-21 (2003). 2.Sweadner, K.J., et al., Genomics 68(1):41-56 (2000). 3.Mounsey, J.P., et al., J. Biol. Chem. 275(30):23362-23367 (2000). 4.Chen, L.S., et al., Genomics 41(3):435-443 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 259490450
• NCBI GeneID: 56188
• NCBI Accession #: NP_062376
• NCBI GenBank Nucleotide #: NP_062376.2
• UniProt Accession #: Q9Z239
• Molecular Weight: 10.323
• NCBI Official Full Name: phospholemman isoform a
• NCBI Official Synonym Full Names: FXYD domain-containing ion transport regulator 1
• NCBI Official Symbol: Fxyd1
• NCBI Official Synonym Symbols: Plm; Pml; 0610012C17Rik; 1110006M24Rik
• NCBI Protein Information: phospholemman
• UniProt Protein Name: Phospholemman
• Protein Family: FXYD domain-containing ion transport regulator
• UniProt Gene Name: Fxyd1
• UniProt Synonym Gene Names: Plm
• UniProt Entry Name: PLM_MOUSE

NCBI Description

This gene encodes a member of the FXYD family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity and act as an accessory protein of Na,K-ATPase. Alternatively spliced transcript variants have been described. [provided by RefSeq, Sep 2009]

Uniprot Description

PLM: a protein of the FXYD family of small ion transport regulators or channels. Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. May have a functional role in muscle contraction. Major plasma membrane substrate for PKA and PKC in several different tissues. Phosphorylated in response to insulin and adrenergic stimulation.Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining.

Protein type: Cell surface; Channel, misc.; Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; cell; plasma membrane; integral to membrane; caveola; sarcolemma

Molecular Function: chloride channel activity; sodium channel regulator activity; myosin binding; ion channel activity; ATPase binding

Biological Process: positive regulation of organic acid transport; cytosolic calcium ion homeostasis; transport; chloride transport; ion transport