Porcine CD 40 Ligand ELISA Kit | CD40L elisa kit

Porcine CD 40 Ligand ELISA Kit

Cat. Num. AAA735282

• Gene Names: CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• Reactivity: Porcine
• Synonyms: CD 40 Ligand; Porcine CD 40 Ligand ELISA Kit; CD40L elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This CD40L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CD40L. This ELISA kit for research use only, not for therapeutic applications!Q
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD40L elisa kit

Principle of the Assay: CD40L ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CD40L. Standards or samples are then added to the microtiter plate wells and CD40L if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CD40L present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CD40L are added to each well to "sandwich" the CD40L immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CD40L and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD40L concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CD40L elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4557433
• NCBI GeneID: 959
• NCBI Accession #: NP_000065.1
• NCBI GenBank Nucleotide #: NM_000074.2
• Molecular Weight: 29,274 Da
• NCBI Official Full Name: CD40 ligand
• NCBI Official Synonym Full Names: CD40 ligand
• NCBI Official Symbol: CD40LG
• NCBI Official Synonym Symbols: IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• NCBI Protein Information: CD40 ligand; CD40-L; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
• UniProt Protein Name: CD40 ligand
• UniProt Gene Name: CD40LG
• UniProt Synonym Gene Names: CD40L; TNFSF5; TRAP; CD40-L; TRAP
• UniProt Entry Name: CD40L_HUMAN

NCBI Description

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: cytokine activity; CD40 receptor binding; tumor necrosis factor receptor binding

Biological Process: B cell proliferation; platelet activation; regulation of immune response; leukocyte adhesion; positive regulation of interleukin-12 production; B cell differentiation; immunoglobulin secretion; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis

Disease: Immunodeficiency With Hyper-igm, Type 1