Sheep T-cell surface glycoprotein CD3 gamma chain ELISA Kit | CD3G elisa kit

Sheep T-cell surface glycoprotein CD3 gamma chain (CD3G) ELISA Kit

Cat. Num. AAA281172

• Reactivity: Sheep
• Synonyms: T-cell surface glycoprotein CD3 gamma chain; Sheep T-cell surface glycoprotein CD3 gamma chain (CD3G) ELISA Kit; CD3G elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sheep CD3G. No significant cross-reactivity or interference between Sheep CD3G and analogues was observed.
• Sequence Length: 182

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for CD3G elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CD3G in samples. An antibody specific for CD3G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCD3G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD3G is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD3G bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 115995
• NCBI GeneID: 443398
• UniProt Accession #: P18439
• Molecular Weight: 16,723 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 gamma chain
• NCBI Official Symbol: CD3G
• NCBI Protein Information: T-cell surface glycoprotein CD3 gamma chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 gamma chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3G

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.