Rabbit anti-Human CDKN3 Polyclonal Antibody | anti-CDKN3 antibody

CDKN3, CT (CDKN3, CDI1, CIP2, KAP, Cyclin-dependent kinase inhibitor 3, CDK2-associated dual-specificity phosphatase, Cyclin-dependent kinase interactor 1, Cyclin-dependent kinase-interacting protein 2, Kinase-associated phosphatase) (FITC)

Cat. Num. AAA6284735

• Gene Names: CDKN3; KAP; CDI1; CIP2; KAP1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CDKN3; Polyclonal Antibody; CT (CDKN3; CDI1; CIP2; KAP; Cyclin-dependent kinase inhibitor 3; CDK2-associated dual-specificity phosphatase; Cyclin-dependent kinase interactor 1; Cyclin-dependent kinase-interacting protein 2; Kinase-associated phosphatase) (FITC); Kinase-associated phosphatase; anti-CDKN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CDKN3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CDKN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183~212 amino acids from the C-terminal region of human CDKN3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CDKN3 Pab in HL-60 cell lysate. CDKN3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-CDKN3 antibody

CDKN3 belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. The gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers.

Product Categories/Family for anti-CDKN3 antibody

Antibodies; Protein Kinase Inhibitors

References

Yeh, C.T., et al., Biochem. Biophys. Res. Commun. 305(2):311-314 (2003). Yeh, C.T., et al., Cancer Res. 60(17):4697-4700 (2000). Lee, S.W., et al., Mol. Cell. Biol. 20(5):1723-1732 (2000). Poon, R.Y., et al., Science 270(5233):90-93 (1995). Hannon, G.J., et al., Proc. Natl. Acad. Sci. U.S.A. 91(5):1731-1735 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 2499769
• NCBI GeneID: 1033
• UniProt Accession #: Q16667; Q53ZU6; Q5U0M4; Q6P1N8; Q99585; Q9BPW7; Q9BY36; Q9C042; Q9C046; Q9C047; Q9C049; Q9C051
• Molecular Weight: 212
• NCBI Official Full Name: Cyclin-dependent kinase inhibitor 3
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 3
• NCBI Official Symbol: CDKN3
• NCBI Official Synonym Symbols: KAP; CDI1; CIP2; KAP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 3; kinase associated phosphatase; kinase-associated phosphatase; Cdk-associated protein phosphatase; cyclin-dependent kinase interactor 1; CDK2-associated dual specificity phosphatase; CDK2-associated dual-specificity pho
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 3
• Protein Family: Cyclin-dependent kinase inhibitor
• UniProt Gene Name: CDKN3
• UniProt Synonym Gene Names: CDI1; CIP2; KAP
• UniProt Entry Name: CDKN3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

CDKN3: May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner. Defects in CDKN3 are found in patients with hepatocellular carcinoma (HCC). Belongs to the protein-tyrosine phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity; EC 3.1.3.16; Cell cycle regulation; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 14q22

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function: protein binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein serine/threonine phosphatase activity

Biological Process: negative regulation of cell proliferation; regulation of cyclin-dependent protein kinase activity; cell cycle arrest; G1/S transition of mitotic cell cycle

Disease: Hepatocellular Carcinoma