CD3e/CD3 epsilon Recombinant Protein | CD3e recombinant protein

Recombinant Cynomolgus CD3e/CD3 epsilon Protein (C-Fc)

Cat. Num. AAA2557560

• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: CD3e/CD3 epsilon; Recombinant Cynomolgus CD3e/CD3 epsilon Protein (C-Fc); CD3 epsilon; CD3e antigen; epsilon polypeptide (TiT3 complex); CD3e molecule; epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex; epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE; CD3e recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 50 mM Tris, 100 mM Glycine, pH 7.5.
• Sequence: Gln22-Asp117
• Sequence Length: 198

• Species: Cynomolgus
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-Fc
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for CD3e recombinant protein

Protein Construction: Recombinant Cynomolgus CD3e is produced by our mammalian expression system and the target gene encoding Gln22-Asp117 is expressed with a Fc tag at the C-terminus.

Background: T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.

NCBI and Uniprot Product Information

• NCBI GI #: 545688608
• NCBI GeneID: 102133065
• NCBI Accession #: NP_001270544.1
• NCBI GenBank Nucleotide #: NP_001270544.1
• UniProt Accession #: Q95LI5
• Molecular Weight: Molecular Mass: 38kDa; Actual Protein Molecular Mass: 38-55kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Symbol: CD3E
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3E

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.