CD3D&CD3E Heterodimer Recombinant Protein | CD3D&CD3E recombinant protein

Recombinant Cynomolgus CD3D&CD3E Heterodimer (C-Fc-DYKDDDDK&C-Fc-6His)

Cat. Num. AAA2569420

• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: CD3D&CD3E Heterodimer; Recombinant Cynomolgus CD3D&CD3E Heterodimer (C-Fc-DYKDDDDK&C-Fc-6His); CD3E & CD3D; CD3 delta & CD3 epsilon; CD3 delta/epsilon; CD3D&CD3E recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Phe22-Ala105&Gln22-Asp117

• Species: Cynomolgus
• Tag: C-Fc-DYKDDDDK&C-Fc-6His
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Cynomolgus monkey CD3D&CD3E Heterodimer is produced by our Mammalian expression system and the target gene encoding Phe22-Ala105&Gln22-Asp117 is expressed with a Fc-DYKDDDDK, Fc-6His tag at the C-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for CD3D&CD3E recombinant protein

T-cell surface glycoprotein CD3D & CD3E, also known as CD3 delta & CD3 epsilon chain, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 567315991
• NCBI GeneID: 102133701
• NCBI Accession #: NP_001274617.1
• NCBI GenBank Nucleotide #: NM_001287688.1
• UniProt Accession #: Q95LI8; Q95LI5
• Molecular Weight: 18,960 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 delta chain
• NCBI Official Symbol: CD3D
• NCBI Protein Information: T-cell surface glycoprotein CD3 delta chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 delta chain
• UniProt Gene Name: CD3D

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.