CD3d/CD3 delta Recombinant Protein | CD3d recombinant protein

Recombinant Cynomolgus CD3d/CD3 delta Protein Protein (C-His)

Cat. Num. AAA2557549

• Purity: >85% as determined by reducing SDS-PAGE.
• Synonyms: CD3d/CD3 delta; Recombinant Cynomolgus CD3d/CD3 delta Protein Protein (C-His); T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; CD3d; CD3D; CD3d recombinant protein

• Host: Human Cells
• Purity/Purification: >85% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: Phe22-Ala105
• Sequence Length: 171

• Species: Cynomolgus
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for CD3d recombinant protein

Protein Construction: Recombinant Cynomolgus CD3d is produced by our mammalian expression system and the target gene encoding Phe22-Ala105 is expressed with a 6His tag at the C-terminus.

Background: T-cell surface glycoprotein CD3 delta chain (CD3D) is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. CD3 chains are present as CD3gammaepsilon, deltaepsilon, and zetazeta dimers in the receptor complex and play critical roles in the antigen receptor assembly, transport to the cell surface, and the receptor-mediated signal transduction. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs(ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A.

NCBI and Uniprot Product Information

• NCBI GI #: 567315991
• NCBI GeneID: 102133701
• NCBI Accession #: NP_001274617.1
• NCBI GenBank Nucleotide #: NP_001274617.1
• UniProt Accession #: Q95LI8
• Molecular Weight: Molecular Mass: 10.4kDa; Actual Protein Molecular Mass: 20-30kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD3 delta chain
• NCBI Official Symbol: CD3D
• NCBI Protein Information: T-cell surface glycoprotein CD3 delta chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 delta chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3D

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.