T-Cell Surface Glycoprotein CD3 epsilon Chain Recombinant Protein | CD3E recombinant protein

Recombinant Human T-Cell Surface Glycoprotein CD3 epsilon Chain Protein

Cat. Num. AAA2556687

• Gene Names: CD3E; T3E; TCRE; IMD18
• Purity: >90% as determined by reducing SDS-PAGE.
• Synonyms: T-Cell Surface Glycoprotein CD3 epsilon Chain; Recombinant Human T-Cell Surface Glycoprotein CD3 epsilon Chain Protein; T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e; CD3E; T3E; CD3E recombinant protein

• Host: Human Cells
• Purity/Purification: >90% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence Length: 207

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Asp23-Asp126
• Fusion Tag: C-6His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

SDS-Page

Related Product Information for CD3E recombinant protein

Protein Construction: Recombinant Human T-Cell Surface Glycoprotein CD3 epsilon Chain is produced by our Mammalian expression system and the target gene encoding Asp23-Asp126 is expressed with a 6His tag at the C-terminus.

Background: T-Cell Surface Glycoprotein CD3 epsilon Chain (CD3epsilon) is a single-pass type I membrane protein. CD3epsilon contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3epsilon is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 4502671
• NCBI GeneID: 916
• NCBI Accession #: NP_000724.1
• NCBI GenBank Nucleotide #: NM_000733.3
• UniProt Accession #: P07766; A8K997
• Molecular Weight: Molecular Mass: 12.8kDa; Actual Protein Molecular Mass: 18kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E

NCBI Description

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).