CD36 / SCARB3 recombinant protein

Recombinant Mouse CD36 / SCARB3 Protein (His tag)

Cat. Num. AAA2546989

• Gene Names: Cd36; FAT; GPIV; Scarb3
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: CD36 / SCARB3; Recombinant Mouse CD36 / SCARB3 Protein (His tag); Cd36; FAT; GPIV; Scarb3; CD36 / SCARB3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 472

• Application Notes: The secreted recombinant mouse CD36 consists of 421 amino acids and has a calculated molecular mass of 47.8 kDa. As a result of glycosylation, the apparent molecular mass of rmCD36 is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gly 30
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD36 / SCARB3 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 36 (CD36), also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb), is a member of the CD system as well as the class B scavenger receptor family of cell surface proteins. CD36 can be found on the surface of many cell type s in vertebrate animals and it consists of 472 amino acids and is extensively glycosylated. It is an integral membrane protein primarily serving as receptors for thrombospondin and collagen and by the erythrocytes infected with the human malaria parasite. The role of CD36 as a cell surface receptor has been extended to that of a signal transduction molecule.

Description: A DNA sequence encoding the extracellular domain of mouse CD36 (NP_001153030.1) (Gly 30-Lys 439) was expressed, with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 227116353
• NCBI GeneID: 12491
• NCBI Accession #: NP_001153030.1
• NCBI GenBank Nucleotide #: NM_001159558.1
• UniProt Accession #: Q08857
• Molecular Weight: 52,698 Da
• NCBI Official Full Name: platelet glycoprotein 4
• NCBI Official Synonym Full Names: CD36 antigen
• NCBI Official Symbol: Cd36
• NCBI Official Synonym Symbols: FAT; GPIV; Scarb3
• NCBI Protein Information: platelet glycoprotein 4
• UniProt Protein Name: Platelet glycoprotein 4
• UniProt Gene Name: Cd36
• UniProt Synonym Gene Names: GPIIIB; GPIV

Uniprot Description

CD36: Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7). Belongs to the CD36 family.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5 A3|5 8.11 cM

Cellular Component: apical part of cell; brush border membrane; caveola; cell surface; endoplasmic reticulum; external side of plasma membrane; extracellular space; Golgi apparatus; intracellular; intracellular membrane-bound organelle; lipid raft; membrane; mitochondrion; plasma membrane; protein complex; sarcolemma

Molecular Function: high-density lipoprotein binding; lipid binding; low-density lipoprotein binding; low-density lipoprotein receptor activity; protein binding; transforming growth factor beta binding

Biological Process: apoptotic cell clearance; cell surface receptor linked signal transduction; cGMP-mediated signaling; cholesterol absorption; cholesterol transport; defense response to Gram-positive bacterium; eating behavior; elevation of cytosolic calcium ion concentration; fatty acid metabolic process; fatty acid oxidation; fatty acid transport; glucose homeostasis; interleukin-1 beta secretion; intestinal absorption; lipoprotein transport; long-chain fatty acid metabolic process; long-chain fatty acid transport; low density lipoprotein mediated signaling; negative regulation of angiogenesis; negative regulation of systemic arterial blood pressure; negative regulation of transcription factor import into nucleus; negative regulation of transcription from RNA polymerase II promoter; nitric oxide mediated signal transduction; phagocytosis, recognition; positive regulation of blood coagulation; positive regulation of cell-matrix adhesion; positive regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; positive regulation of MAPKKK cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phagocytosis; positive regulation of phagocytosis, engulfment; positive regulation of tumor necrosis factor production; receptor internalization; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; response to lipid; response to mechanical stimulus; sensory perception of taste; sequestering of lipid; triacylglycerol metabolic process; triacylglycerol transport