CD1E recombinant protein

CD1E, 32- 305aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205641

• Gene Names: CD1E; R2; CD1A
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: CD1E; 32- 305aa; Human; His tag; E Coli (Denatured); T-cell surface glycoprotein CD1e; CD1A; R2; CD1E recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 0.4M urea
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSEEQLSFR MLQTSSFANH SWAHSEGSGW LGDLQTHGWD TVLGTIRFLK PWSHGNFSKQ ELKNLQSLFQ LYFHSFIQIV QASAGQFQLE YPFEIQILAG CRMNAPQIFL NMAYQGSDFL SFQGISWEPS PGAGIRAQNI CKVLNRYLDI KEILQSLLGH TCPRFLAGLM EAGESELKRK VKPEAWLSCG PSPGPGRLQL VCHVSGFYPK PVWVMWMRGE QEQRGTQRGD VLPNADETWY LRATLDVAAG EAAGLSCRVK HSSLGGHDLI IHWGGYS
• Sequence Length: 388

• Applicable Applications for CD1E recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CD1E recombinant protein

CD1E is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. CD1E localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members. Recombinant human CD1E protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for CD1E recombinant protein

Immunology

References

Angenieux C., et al (2000). J. Biol. Chem. 275:37757-37764; de la Salle H., et al (2005). Science 310:1321-1324;

NCBI and Uniprot Product Information

• NCBI GI #: 110618240
• NCBI GeneID: 913
• NCBI Accession #: NP_112155
• NCBI GenBank Nucleotide #: NM_030893.3
• UniProt Accession #: P15812; Q5TDJ9; Q5TDK3; Q5TDK4; Q5TDK5; Q5TDK6; Q5TDK8; Q5TDL1; Q712E4; Q712E5; B4DZV3; E7EP01
• Molecular Weight: 33.1 kDa (297aa)
• NCBI Official Full Name: T-cell surface glycoprotein CD1e, membrane-associated isoform a
• NCBI Official Synonym Full Names: CD1e molecule
• NCBI Official Symbol: CD1E
• NCBI Official Synonym Symbols: R2; CD1A
• NCBI Protein Information: T-cell surface glycoprotein CD1e, membrane-associated
• UniProt Protein Name: T-cell surface glycoprotein CD1e, membrane-associated
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD1E
• UniProt Synonym Gene Names: hCD1e; sCD1e

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members. Many alternatively spliced transcript variants encoding different isoforms have been described. Additional transcript variants have been found; however, their biological validity has not been determined. [provided by RefSeq, Jun 2010]

Uniprot Description

T-cell surface glycoprotein CD1e, soluble binds diacetylated lipids, including phosphatidyl inositides and diacylated sulfoglycolipids, and is required for the presentation of glycolipid antigens on the cell surface. The membrane-associated form is not active.