Rabbit anti-Human CD1E Polyclonal Antibody | anti-CD1E antibody

CD1E, ID (CD1E, T-cell surface glycoprotein CD1e, membrane-associated, R2G1, CD1e, T-cell surface glycoprotein CD1e, soluble) (Azide free) (HRP)

Cat. Num. AAA6282898

• Gene Names: CD1E; R2; CD1A
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD1E; Polyclonal Antibody; ID (CD1E; T-cell surface glycoprotein CD1e; membrane-associated; R2G1; CD1e; soluble) (Azide free) (HRP); soluble; anti-CD1E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CD1E antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CD1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human CD1E.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CD1E Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CD1E antibody detected the CD1E protein (arrow).)

Immunohistochemistry (IHC)

(CD1E Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD1E Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CD1E Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CD1E Antibody (Center) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-CD1E antibody

CD1E encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members.

Product Categories/Family for anti-CD1E antibody

Antibodies; CD Markers

References

Maitre, B., et al. Biochem. J. 419(3):661-668(2009). Kuijf, M.L., et al. J. Neuroimmunol. 205 (1-2), 110-112 (2008). Maitre, B., et al. Traffic 9(4):431-445(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 110618240
• NCBI GeneID: 913
• NCBI Accession #: NP_112155.2
• NCBI GenBank Nucleotide #: NM_030893.3
• UniProt Accession #: P15812; Q5TDJ9; Q5TDK3; Q5TDK4; Q5TDK5; Q5TDK6; Q5TDK8; Q5TDL1; Q712E4; Q712E5; B4DZV3; E7EP01
• Molecular Weight: 32,303 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD1e, membrane-associated isoform a
• NCBI Official Synonym Full Names: CD1e molecule
• NCBI Official Symbol: CD1E
• NCBI Official Synonym Symbols: R2; CD1A
• NCBI Protein Information: T-cell surface glycoprotein CD1e, membrane-associated
• UniProt Protein Name: T-cell surface glycoprotein CD1e, membrane-associated
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD1E
• UniProt Synonym Gene Names: hCD1e; sCD1e
• UniProt Entry Name: CD1E_HUMAN

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes within Golgi compartments, endosomes, and lysosomes, and is cleaved into a stable soluble form. The soluble form is required for the intracellular processing of some glycolipids into a form that can be presented by other CD1 family members. Many alternatively spliced transcript variants encoding different isoforms have been described. Additional transcript variants have been found; however, their biological validity has not been determined. [provided by RefSeq, Jun 2010]

Uniprot Description

CD1E: T-cell surface glycoprotein CD1e, soluble is required for the presentation of glycolipid antigens on the cell surface. The membrane-associated form is not active. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: Golgi membrane; lysosomal lumen; integral to plasma membrane; late endosome; early endosome; plasma membrane

Molecular Function: beta-2-microglobulin binding; exogenous lipid antigen binding; endogenous lipid antigen binding

Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; immune response