Rabbit anti-Human PDGFA Polyclonal Antibody | anti-PDGFA antibody

PDGFA, NT (Platelet-derived Growth Factor Subunit A, PDGF Subunit A, Platelet-derived Growth Factor A Chain, Platelet-derived Growth Factor alpha Polypeptide, PDGF-1, PDGF1) (Biotin)

Cat. Num. AAA6493769

• Gene Names: PDGFA; PDGF1; PDGF-A
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: PDGFA; Polyclonal Antibody; NT (Platelet-derived Growth Factor Subunit A; PDGF Subunit A; Platelet-derived Growth Factor A Chain; Platelet-derived Growth Factor alpha Polypeptide; PDGF-1; PDGF1) (Biotin); anti-PDGFA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDGFA.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PDGFA antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 60-89 in the N-terminal region of human PDGFA, UniProt Accession #P04085
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis to detect PDGFA in HL60 cell lysate usingMBS6008287.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted withMBS6008287, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6008287 for immunohistochemistry.)

Related Product Information for anti-PDGFA antibody

PDGFA is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth.

Product Categories/Family for anti-PDGFA antibody

Antibodies; Growth Factors; Cytokines

References

1. Monje, P., et al., Mol. Cell. Biol. 23(19):7030-7043 (2003). 2. Gianni, D., et al., J. Biol. Chem. 278(11):9290-9297 (2003). 3. Muller, C., et al., J. Biol. Chem. 278(20):18330-18335 (2003). 4. Chui, C.M., et al., Cytokine 21(2):51-64 (2003). 5. Laprise, M.H., et al., Blood 100(10):3578-3587 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 77695917
• NCBI GeneID: 5154
• NCBI Accession #: NP_002598
• NCBI GenBank Nucleotide #: NM_002607.5
• UniProt Accession #: P04085; B5BU73
• Molecular Weight: 22,253 Da
• NCBI Official Full Name: platelet-derived growth factor subunit A isoform 1 preproprotein
• NCBI Official Synonym Full Names: platelet derived growth factor subunit A
• NCBI Official Symbol: PDGFA
• NCBI Official Synonym Symbols: PDGF1; PDGF-A
• NCBI Protein Information: platelet-derived growth factor subunit A
• UniProt Protein Name: Platelet-derived growth factor subunit A
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFA
• UniProt Synonym Gene Names: PDGF1; PDGF subunit A
• UniProt Entry Name: PDGFA_HUMAN

NCBI Description

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Cell cycle regulation; Secreted

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: Golgi membrane; extracellular space; microvillus; cell surface; endoplasmic reticulum lumen; extracellular region

Molecular Function: collagen binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding

Biological Process: skin development; extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; wound healing; negative chemotaxis; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; response to estradiol stimulus; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; regulation of smooth muscle cell migration; platelet degranulation; hair follicle development; positive regulation of MAPKKK cascade; cell-cell signaling; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to wounding; angiogenesis; inner ear development; regulation of peptidyl-tyrosine phosphorylation; epidermal growth factor receptor signaling pathway; response to drug; platelet activation; response to inorganic substance; phosphoinositide-mediated signaling; response to retinoic acid; fibroblast growth factor receptor signaling pathway; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; cell activation; organ morphogenesis; regulation of actin cytoskeleton organization and biogenesis; positive regulation of cell division; response to hypoxia; innate immune response; actin cytoskeleton organization and biogenesis; blood coagulation; alveolus development; positive regulation of DNA replication; positive regulation of cell migration