Mouse anti-Human CCT5 / TCP1 Epsilon Monoclonal Antibody | anti-CCT5 antibody

Mouse Monoclonal [clone 4E5-4B1] (IgG1,k) to Human CCT5 / TCP1 Epsilon

Cat. Num. AAA246081

• Gene Names: CCT5; CCTE; HEL-S-69; CCT-epsilon; TCP-1-epsilon
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: CCT5 / TCP1 Epsilon; Monoclonal Antibody; Mouse Monoclonal [clone 4E5-4B1] (IgG1; k) to Human CCT5 / TCP1 Epsilon; Anti-CCT5 / TCP1 Epsilon Antibody (clone 4E5-4B1) IHC-plus; CCT5; KIAA0098; CCT-epsilon; CCTE; TCP-1-epsilon; TCP1 Epsilon; Human CCT5; anti-CCT5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4E5-4B1
• Specificity: Human CCT5
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 0.388 mg/ml (varies by lot)
• Sequence Length: 541

• Applicable Applications for anti-CCT5 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: CCT5 (AAH06543, 1 a.a. ~ 542 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: CCT5 / TCP1 Epsilon antibody was raised against cCT5 (AAH06543, 1 a.a. ~ 542 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-CCT5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CCT5 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to CCT5 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western blot of CCT5 expression in HeLa cells with CCT5 monoclonal antibody, clone 4E5-4B1.)

ELISA (EIA)

(Detection limit for recombinant GST tagged CCT5 is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 24307939
• NCBI GeneID: 22948
• NCBI Accession #: NP_036205.1
• NCBI GenBank Nucleotide #: NM_012073.3
• UniProt Accession #: P48643; A8JZY8; A8K2X8; B4DYD8
• Molecular Weight: 49,526 Da
• NCBI Official Full Name: T-complex protein 1 subunit epsilon
• NCBI Official Synonym Full Names: chaperonin containing TCP1, subunit 5 (epsilon)
• NCBI Official Symbol: CCT5
• NCBI Official Synonym Symbols: CCTE; HEL-S-69; CCT-epsilon; TCP-1-epsilon
• NCBI Protein Information: T-complex protein 1 subunit epsilon; epididymis secretory protein Li 69; T-complex protein 1, epsilon subunit
• UniProt Protein Name: T-complex protein 1 subunit epsilon
• Protein Family: T-complex protein
• UniProt Gene Name: CCT5
• UniProt Synonym Gene Names: CCTE; KIAA0098; TCP-1-epsilon
• UniProt Entry Name: TCPE_HUMAN

NCBI Description

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. In addition, three pseudogenes of this gene have been identified. [provided by RefSeq, Jun 2010]

Uniprot Description

CCT-epsilon: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Defects in CCT5 are the cause of hereditary sensory neuropathy autosomal recessive with spastic paraplegia (HSNSP). The disease is characterized by spastic paraplegia and progressive distal sensory neuropathy leading to mutilating ulcerations of the upper and lower limbs. Belongs to the TCP-1 chaperonin family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: chaperonin-containing T-complex; microtubule; centrosome; cytoplasm; nucleolus; cytosol

Molecular Function: protein binding; unfolded protein binding; beta-tubulin binding; G-protein beta-subunit binding; ATP binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; response to virus

Disease: Neuropathy, Hereditary Sensory, With Spastic Paraplegia, Autosomal Recessive