CCNC recombinant protein
Recombinant Human Cyclin-C
Gene Names
CCNC; CycC
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Greater than 98% as determined by SDS-PAGE.
Synonyms
CCNC; Recombinant Human Cyclin-C; Cyclin C Human Recombinant; CCNC Human; Cyclin C.; CCNC recombinant protein
Host
E Coli
Purity/Purification
Greater than 98% as determined by SDS-PAGE.
Form/Format
CCNC is supplied at 0.1mg/ml in Glycerol, TCEP, DTT, SDS, 1.0M Tris-HCl pH 6.7 and bromophenol blue.
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSEILPY SEDKMGRFGA DPEGSDLSFS CRLQDTNSFF AGNQAKRPPK LGQIGRAKRV VIEDDRIDDV LKGMGEKPPS GV.
Sequence Length
198
Applicable Applications for CCNC recombinant protein
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
CCNC can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 1-2 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.
Related Product Information for CCNC recombinant protein
Cyclin C Recombinant is expressed in E.coli as a 35kDa tagged fusion protein corresponding to amino acids 120-303 of Cyclin C of human origin. Cyclin C is purified from bacterial lysates by glutathione agarose affinity chromatography.
Introduction: CCNC (cyclin C) is a member of the cyclin family of proteins. This protein interacts with cyclin-dependent kinase 8 (CDK8) and induces the phosphorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this protein peaks in the G1 phase of the cell cycle. It has been shown that a non-cdk8-associated cellular pool of CCNC combines with cdk3 to stimulate pRb phosphorylation at S807/811 during the G0/G1 transition, and that this phosphorylation is required for cells to exit GO efficiently. Other results have shown that a marked elevated immunoreactivity of CCNC was found both in neurons and astrocytes in Alzheimer's disease (AD). Increased levels of CCNC RNA were detected by ribonuclease protection assay (RPA) in severe AD cases and colocalization of CCNC and its preferred binding partner, CDK8, was only observed in astrocytes but not in neurons.
Introduction: CCNC (cyclin C) is a member of the cyclin family of proteins. This protein interacts with cyclin-dependent kinase 8 (CDK8) and induces the phosphorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this protein peaks in the G1 phase of the cell cycle. It has been shown that a non-cdk8-associated cellular pool of CCNC combines with cdk3 to stimulate pRb phosphorylation at S807/811 during the G0/G1 transition, and that this phosphorylation is required for cells to exit GO efficiently. Other results have shown that a marked elevated immunoreactivity of CCNC was found both in neurons and astrocytes in Alzheimer's disease (AD). Increased levels of CCNC RNA were detected by ribonuclease protection assay (RPA) in severe AD cases and colocalization of CCNC and its preferred binding partner, CDK8, was only observed in astrocytes but not in neurons.
Product Categories/Family for CCNC recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,243 Da
NCBI Official Full Name
cyclin-C isoform b
NCBI Official Synonym Full Names
cyclin C
NCBI Official Symbol
CCNC
NCBI Official Synonym Symbols
CycC
NCBI Protein Information
cyclin-C; hSRB11; SRB11 homolog
UniProt Protein Name
Cyclin-C
UniProt Gene Name
CCNC
UniProt Synonym Gene Names
hSRB11
UniProt Entry Name
CCNC_HUMAN
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Product Notes
The CCNC ccnc (Catalog #AAA142805) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CCNC can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). CCNC can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested. Researchers should empirically determine the suitability of the CCNC ccnc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHH HHH SSGLVPRGSH MGSMSE ILPY SEDKMGRFGA DPEGSDLSFS CRLQDTNSFF AGNQAKRPPK LGQIGRAKRV VIEDDRIDDV LKGMGEKPPS GV.. It is sometimes possible for the material contained within the vial of "CCNC, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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