CCL8 / MCP-2 recombinant protein

Human CCL8 / MCP-2 Protein (His Tag)

Cat. Num. AAA2545135

• Gene Names: CCL8; HC14; MCP2; MCP-2; SCYA8; SCYA10
• Purity: > 95 % as determined by SDS-PAGE.
• Synonyms: CCL8 / MCP-2; Human CCL8 / MCP-2 Protein (His Tag); MCP2; SCYA10; SCYA8; CCL8 / MCP-2 recombinant protein

• Host: Yeast
• Purity/Purification: > 95 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 99

• Application Notes: The recombinant human CCL8 consists of 86 amino acids and predicts a molecular mass of 10.3 kDa.
• Predicted N Terminal: Gln 24
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CCL8 / MCP-2 recombinant protein

Background: Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 8 (CCL8), also known as monocyte chemoattractant protein 2 (MCP-2), is a small cytokine belonging to the C-C chemokine family. CCL8 functions to activate different immune cells, including mast cells, eosinophils and basophils which are involved in allergic responses, monocytes, and T cells and NK cells which are involved in the inflammatory response. CCL8's ability achieves by binding to different cell surface receptors termed chemokine receptors including CCR1, CCR2B and CCR5. It has been reported that CCL8 is a potent inhibitor of HIV-1 by virtue of its binding to CCR5 which is one of the major co-receptors for HIV-1.

Description: A DNA sequence encoding the human CCL8 (NP_005614.2) (Gln24-Pro99) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 22538816
• NCBI GeneID: 6355
• NCBI Accession #: NP_005614.2
• NCBI GenBank Nucleotide #: NM_005623.2
• UniProt Accession #: P80075; P78388; A0AV77
• Molecular Weight: 11,246 Da
• NCBI Official Full Name: C-C motif chemokine 8
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 8
• NCBI Official Symbol: CCL8
• NCBI Official Synonym Symbols: HC14; MCP2; MCP-2; SCYA8; SCYA10
• NCBI Protein Information: C-C motif chemokine 8
• UniProt Protein Name: C-C motif chemokine 8
• UniProt Gene Name: CCL8
• UniProt Synonym Gene Names: MCP2; SCYA10; SCYA8; MCP-2

NCBI Description

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: CCR chemokine receptor binding; phospholipase activator activity; protein kinase activity

Biological Process: calcium ion transport; cell-cell signaling; cellular calcium ion homeostasis; chemotaxis; exocytosis; G-protein coupled receptor protein signaling pathway; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of GTPase activity; positive regulation of inflammatory response; response to virus; signal transduction