Typical Testing Data/Standard Curve (for reference only)
Mouse chemokine (C-C motif) ligand 3 ELISA Kit | CCL3 elisa kit
Mouse Macrophage Inflammatory Protein 1alpha, MIP-1alpha ELISA Kit
Gene Names
Ccl3; Mip1a; Scya3; G0S19-1; AI323804; MIP1-(a); LD78alpha; MIP-1alpha; MIP1-alpha
Reactivity
Mouse
Synonyms
chemokine (C-C motif) ligand 3; Mouse Macrophage Inflammatory Protein 1alpha; MIP-1alpha ELISA Kit; MIP-1alpha ELISA kit; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3; LD78 alpha beta; small inducible cytokine A3 (homologous to mouse Mip-1a) ; CCL3 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CCL3. No significant cross-reactivity or interference between Mouse CCL3 and analogues was observed.
Sequence Length
92
Samples
Serum, plasma and tissue homogenates
Assay Type
Sandwich
Detection Range
23.5 pg/ml-1500 pg/ml
Sensitivity
5.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Immunity
Protein Biological Process 2
chemokine
Protein Biological Process 3
Chemotaxis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CCL3 elisa kit
For Sample For the quantitative determination of mouse macrophage inflammatory protein 1? (MIP-1?) concentrations in serum, plasma, tissue homogenates.
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIP-1? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-1? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIP-1? is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIP-1? bound in the initial step. The color development is stopped and the intensity of the color is measured.
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIP-1? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-1? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIP-1? is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIP-1? bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,345 Da
NCBI Official Full Name
C-C motif chemokine 3
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 3
NCBI Official Symbol
Ccl3
NCBI Official Synonym Symbols
Mip1a; Scya3; G0S19-1; AI323804; MIP1-(a); LD78alpha; MIP-1alpha; MIP1-alpha
NCBI Protein Information
C-C motif chemokine 3; TY-5; L2G25B; MIP1 (a); SIS-alpha; MIP-1 alpha; MIP-1-alpha; small inducible cytokine A3; small-inducible cytokine A3; heparin-binding chemotaxis protein; macrophage inflammatory protein-1alpha; macrophage inflammatory protein 1-alpha
UniProt Protein Name
C-C motif chemokine 3
Protein Family
UniProt Gene Name
Ccl3
UniProt Synonym Gene Names
Mip1a; Scya3; MIP-1-alpha
UniProt Entry Name
CCL3_MOUSE
Uniprot Description
Function: Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.
Research Articles on CCL3
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Product Notes
The Mouse CCL3 ccl3 (Catalog #AAA701792) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA701792 ELISA Kit recognizes Mouse CCL3. It is sometimes possible for the material contained within the vial of "chemokine (C-C motif) ligand 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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