Eotaxin-2 (CCL24) Recombinant Protein | CCL24 recombinant protein
Mouse Eotaxin-2 (CCL24) Recombinant
Purity
>97%, as determined by SDS-PAGE and HPLC
Synonyms
Eotaxin-2 (CCL24); Mouse Eotaxin-2 (CCL24) Recombinant; Mouse Eotaxin-2 recombinant; ccl24; eotaxin-2; eotaxin2; CCL24 recombinant protein
Host
E Coli
Purity/Purification
>97%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant mouse Eotaxin-2 was lyophilzed from 0.2um filtered concentrated (1.0mg/ml) solution in 100mM NaCl, 10mM PB, pH 7.0.
Sequence
AGVIFITKKG HKICTDPKLL WVQRHIQKLD AKKNQPSKGA KAVRTKFAVQ RRRGNSTEV
Sequence Length
119
Domain
IL8
Host Note
Optimized DNA sequence encoding mouse Eotaxin-2 mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native Mouse Eotaxin-2/CCL24 has a calculated molecular mass of approximately 12 kDa.
Recombinant Eotaxin-2/CCL24 is a disulfide-linked homodimeric protein consisting of 94 amino acid residue subunits, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Recombinant Eotaxin-2/CCL24 is a disulfide-linked homodimeric protein consisting of 94 amino acid residue subunits, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Related Product Information for CCL24 recombinant protein
Eotaxin belongs to the platelet factor-4 family of chemokines. The gene symbol is SCYA11. The factor is being referred to also as Eotaxin-1 and has been renamed CCL11. Eotaxin induces substantial accumulation of eosinophils in the skin without significantly affecting the accumulation of neutrophils. Eotaxin is a potent stimulator of both guinea pig and human eosinophils in vitro. The Eotaxin receptor is a G-protein-coupled receptor selectively expressed in human eosinophils.
Product Categories/Family for CCL24 recombinant protein
References
• Eotaxin-2 and colorectal cancer: a potential target for immune therapy .Clin. Cancer Res., Oct 2007; 13: 5719 - 5728.
• coexpression of il-5 and Eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma .J. Immunol., Jun 2007; 178: 7879 - 7889.
• Eotaxin-2 in sputum cell culture to evaluate asthma inflammation .Eur. Respir. J., Mar 2007; 29: 489 - 495.
• identification of a cooperative mechanism involving interleukin-13 and Eotaxin-2 in experimental allergic lung inflammation .J. Biol. Chem., Apr 2005; 280: 13952 - 13961.
• eotaxin (ccl11) and Eotaxin-2 (ccl24) induce recruitment of eosinophils, basophils, neutrophils, and macrophages as well as features of early- and late-phase allergic reactions following cutaneous injection in human atopic and nonatopic volunteers .J. Immunol., Sep 2002; 169: 2712 - 2718.
• coexpression of il-5 and Eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma .J. Immunol., Jun 2007; 178: 7879 - 7889.
• Eotaxin-2 in sputum cell culture to evaluate asthma inflammation .Eur. Respir. J., Mar 2007; 29: 489 - 495.
• identification of a cooperative mechanism involving interleukin-13 and Eotaxin-2 in experimental allergic lung inflammation .J. Biol. Chem., Apr 2005; 280: 13952 - 13961.
• eotaxin (ccl11) and Eotaxin-2 (ccl24) induce recruitment of eosinophils, basophils, neutrophils, and macrophages as well as features of early- and late-phase allergic reactions following cutaneous injection in human atopic and nonatopic volunteers .J. Immunol., Sep 2002; 169: 2712 - 2718.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,871 Da
NCBI Official Full Name
C-C motif chemokine 24
UniProt Protein Name
C-C motif chemokine 24
Protein Family
UniProt Gene Name
Ccl24
UniProt Synonym Gene Names
Scya24
UniProt Entry Name
CCL24_MOUSE
Uniprot Description
CCL24: Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Cellular Component: extracellular space; extracellular region
Molecular Function: chemokine activity; cytokine activity
Biological Process: regulation of cell shape; positive regulation of angiogenesis; positive regulation of actin filament polymerization; eosinophil chemotaxis; cytoskeleton organization and biogenesis; positive regulation of endothelial cell proliferation; immune response; chemotaxis; inflammatory response; positive regulation of cell migration; positive regulation of inflammatory response
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Product Notes
The CCL24 ccl24 (Catalog #AAA553266) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: AGVIFITKKG HKICTDPKLL WVQRHIQKLD AKKNQPSKGA KAVRTKFAVQ RRRGNSTEV. It is sometimes possible for the material contained within the vial of "Eotaxin-2 (CCL24), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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