Mouse chemokine (C-C motif) ligand 19 ELISA Kit | CCL19 elisa kit

Mouse macrophage inflammatory protein 3beta, MIP-3beta ELISA Kit

Cat. Num. AAA701440

• Gene Names: Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3
• Reactivity: Mouse
• Synonyms: chemokine (C-C motif) ligand 19; Mouse macrophage inflammatory protein 3beta; MIP-3beta ELISA Kit; MIP-3beta ELISA kit; RP11-195F19.11; CKb11; ELC; MGC34433; MIP-3b; MIP3B; SCYA19; CC chemokine ligand 19; CK beta-11; EBI1-ligand chemokine; beta chemokine exodus-3; exodus-3; macrophage inflammatory protein 3-beta; small ind; CCL19 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse MIP-3beta. No significant cross-reactivity or interference between mouse MIP-3beta and analogues was observed.
• Sequence Length: 108

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 7.8 pg/ml -500 pg/ml.
• Sensitivity: The minimum detectable dose of mouse MIP-3beta is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL19 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIP-3beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-3beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIP-3beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIP-3beta bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6755422
• NCBI GeneID: 24047
• NCBI Accession #: NP_036018.1
• NCBI GenBank Nucleotide #: NM_011888.2
• UniProt Accession #: O70460
• Molecular Weight: 11,911 Da
• NCBI Official Full Name: C-C motif chemokine 19
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 19
• NCBI Official Symbol: Ccl19
• NCBI Official Synonym Symbols: ELC; CKb11; MIP3B; Scya19; exodus-3
• NCBI Protein Information: C-C motif chemokine 19; chemokine CCL19; EBI1 ligand chemokine; EBI1-ligand chemokine; EBI-1 ligand chemokine; small inducible cytokine A19; small-inducible cytokine A19; EBV-induced molecule 1 ligand chemokine; epstein-Barr virus-induced molecule 1 ligand chemokine
• UniProt Protein Name: C-C motif chemokine 19
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl19
• UniProt Synonym Gene Names: Elc; Scya19; EBI1 ligand chemokine; ELC
• UniProt Entry Name: CCL19_MOUSE

Uniprot Description

Function: Strongly chemotactic for naive (L-selectinhi) CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.