Human Macrophage Inflammatory Protein 3beta ELISA Kit | MIP-3beta elisa kit

Human Macrophage Inflammatory Protein 3beta ELISA Kit

Cat. Num. AAA764318

• Gene Names: CCL19; ELC; CKb11; MIP3B; MIP-3b; SCYA19
• Reactivity: Human
• Synonyms: Macrophage Inflammatory Protein 3beta; Human Macrophage Inflammatory Protein 3beta ELISA Kit; MIP-3b/ELC/CCL19/beta chemokine exodus-3/Beta-chemokine exodus-3/CC chemokine ligand 19/C-C motif chemokine 19/chemokine (C-C motif) ligand 19/CKb11/EBI1-ligand chemokine/ELCMIP-3-beta/Epstein-Barr virus-induced molecule 1 ligand chemokine/exodus-3/Macrophage inflammatory protein 3 beta/macrophage inflammatory protein 3-beta/MGC34433/MIP-3b/MIP3BCK beta-11/SCYA19EBI1 ligand chemokine/small inducible cytokine subfamily A (Cys-Cys); member 19/Small-inducible cytokine A19; Macrophage Inflammatory Protein 3b; MIP-3beta elisa kit

• Reactivity: Human
• Sequence Length: 98

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MIP-3beta elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MIP-3betaantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MIP-3beta antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MIP-3beta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MIP-3betacan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1791003
• NCBI GeneID: 6363
• Molecular Weight: 10,993 Da
• NCBI Official Full Name: macrophage inflammatory protein 3 beta
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 19
• NCBI Official Symbol: CCL19
• NCBI Official Synonym Symbols: ELC; CKb11; MIP3B; MIP-3b; SCYA19
• NCBI Protein Information: C-C motif chemokine 19
• UniProt Protein Name: C-C motif chemokine 19
• UniProt Gene Name: CCL19
• UniProt Synonym Gene Names: ELC; MIP3B; SCYA19; EBI1 ligand chemokine; ELC; MIP-3-beta
• UniProt Entry Name: CCL19_HUMAN

NCBI Description

This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: cell; extracellular region; extracellular space; intracellular

Molecular Function: CCR chemokine receptor binding; CCR10 chemokine receptor binding; CCR7 chemokine receptor binding; chemokine activity; chemokine receptor binding

Biological Process: activation of JNK activity; cell communication; cell maturation; cellular calcium ion homeostasis; dendritic cell chemotaxis; establishment of T cell polarity; formation of immunological synapse; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; myeloid dendritic cell chemotaxis; neutrophil chemotaxis; positive regulation of chemotaxis; positive regulation of dendritic cell antigen processing and presentation; positive regulation of endocytosis; positive regulation of GTPase activity; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; positive regulation of receptor-mediated endocytosis; positive regulation of T cell proliferation; positive regulation of T-helper 1 cell differentiation; positive regulation of tumor necrosis factor production; release of sequestered calcium ion into cytosol; response to virus; T cell costimulation