Mouse anti-Human TIGIT Monoclonal Antibody | anti-TIGIT antibody

TIGIT Antibody [10B1] (biotin)

Cat. Num. AAA154627

• Gene Names: TIGIT; VSIG9; VSTM3; WUCAM
• Reactivity: Human
• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence
• Purity: protein A purified IgG1
• Synonyms: TIGIT; Monoclonal Antibody; TIGIT Antibody [10B1] (biotin); TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; WUCAM; anti-TIGIT antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10B1
• Purity/Purification: protein A purified IgG1
• Form/Format: Liquid; TIGIT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 244

• Applicable Applications for anti-TIGIT antibody: Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluoresecence (IF)
• Application Notes: TIGIT antibody can be used for immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 1 ug/mL. For flow cytometry at 1 ug/ml. For immunocytochemistry at 1 ug/mL. Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
• Immunogen: TIGIT antibody was raised against the extracellular domain of human TIGIT
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.; As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Immunocytochemistry (IHC)

(Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 ug/ml.Green: TIGIT Antibody [10B1] (MBS154627)Blue: DAPI staining)

Immunohistochemistry (IHC)

(Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 ug/ml.)

Flow cytometry (FC)

(Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 ug/ml.Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)

ELISA

(Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA with MBS154588, MBS154590, MBS154591, MBS154592, MBS154593 and MBS154594 antibodies at decreasing concentrations.)

Related Product Information for anti-TIGIT antibody

The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).

Product Categories/Family for anti-TIGIT antibody

Immunology

References

1. Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63. 2. Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8(+) T cell effector function. Cancer Cell 2014; 26:923-37.

NCBI and Uniprot Product Information

• NCBI GI #: 256600228
• NCBI GeneID: 201633
• NCBI Accession #: NP_776160
• NCBI GenBank Nucleotide #: NM_173799.4
• UniProt Accession #: Q495A1; Q495A3; Q5JPD8; Q6MZS2; Q8N877
• Molecular Weight: Predicted: 26 kDa; Observed: 47 kDa
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: TIGIT
• NCBI Official Synonym Symbols: VSIG9; VSTM3; WUCAM
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• Protein Family: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: TIGIT
• UniProt Synonym Gene Names: VSIG9; VSTM3

NCBI Description

This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]

Uniprot Description

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.