SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CBL recombinant protein
CBL Protein
Applications
Western Blot
Synonyms
CBL; CBL Protein; C-CBL; CBL2; FRA11B; NSLL; RNF55; CBL recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF and 25% glycerol.
Applicable Applications for CBL recombinant protein
Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for CBL recombinant protein
Recombinant human CBL (1-375) was expressed in E Coli cells using an N-terminal GST tag. The gene accession number is NM_005188.
CBL proto-oncogene acts as a RING finger E3 ubiquitin ligase, transfers ubiquitin from specific E2 ubiquitin-conjugating enzymes to targeting substrates for degradation by the proteasome(1). The N-terminal phosphotyrosine binding domain of CBL allows the interaction with the substrates containing phosphorylated tyrosine including the activated receptor tyrosine kinases, which plays the negative regulation of these pathways (1). Mutation or translocation of CBL is involved in acute myeloid leukemia, Jacobsen syndrome and Noonan syndrome-like disorder (2, 3).
CBL proto-oncogene acts as a RING finger E3 ubiquitin ligase, transfers ubiquitin from specific E2 ubiquitin-conjugating enzymes to targeting substrates for degradation by the proteasome(1). The N-terminal phosphotyrosine binding domain of CBL allows the interaction with the substrates containing phosphorylated tyrosine including the activated receptor tyrosine kinases, which plays the negative regulation of these pathways (1). Mutation or translocation of CBL is involved in acute myeloid leukemia, Jacobsen syndrome and Noonan syndrome-like disorder (2, 3).
Product Categories/Family for CBL recombinant protein
References
1. Howlett C.J., et.al. The proto-oncogene p120 (Cbl) is a downstream substrate of the Hck protein-tyrosine kinase. Biochem. Biophys. Res. Commun. 257:129-138, 1999.
2. Muraoka M, et.al. Adults with germline CBL mutation complicated with juvenile myelomonocytic leukemia at infancy. J Hum Genet. Jun; 61(6): 523-6, 2016.
3. Martinelli S., et.al. Heterozygous germline mutations in the CBL tumor-suppressor gene cause a Noonan syndrome-like phenotype. Am. J. Hum. Genet. 87:250-257, 2010.
2. Muraoka M, et.al. Adults with germline CBL mutation complicated with juvenile myelomonocytic leukemia at infancy. J Hum Genet. Jun; 61(6): 523-6, 2016.
3. Martinelli S., et.al. Heterozygous germline mutations in the CBL tumor-suppressor gene cause a Noonan syndrome-like phenotype. Am. J. Hum. Genet. 87:250-257, 2010.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~69kDa
Protein Family
Similar Products
Product Notes
The CBL (Catalog #AAA516718) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CBL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CBL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CBL, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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