Typical Testing Data/Standard Curve (for reference only)
Guinea Pig PTN ELISA Kit | PTN elisa kit
Guinea pig PTN (Pleiotrophin) ELISA Kit
Gene Names
PTN; HARP; HBNF; HBGF8; NEGF1
Reactivity
Guinea Pig
Synonyms
PTN; Guinea pig PTN (Pleiotrophin) ELISA Kit; PTN elisa kit
Reactivity
Guinea Pig
Specificity
This kit recognizes natural and recombinant Guinea pig PTN. No significant cross-reactivity or interference between Guinea pig PTN and analogues was observed.
Sequence Length
168
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PTN elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Guinea pig PTN concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PTN. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PTN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PTN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PTN. You can calculate the concentration of PTN in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PTN. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PTN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PTN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PTN. You can calculate the concentration of PTN in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,942 Da
NCBI Official Full Name
pleiotrophin
NCBI Official Synonym Full Names
pleiotrophin
NCBI Official Symbol
PTN
NCBI Official Synonym Symbols
HARP; HBNF; HBGF8; NEGF1
NCBI Protein Information
pleiotrophin; HB-GAM; HBBM; HBGF-8; HBNF-1; OSF-1; heparin affin regulatory protein; heparin binding growth factor 8; heparin-binding brain mitogen; heparin-binding growth factor 8; heparin-binding growth-associated molecule; heparin-binding neurite outgrowth-promoting factor 1; neurite growth-promoting factor 1; osteoblast-specific factor 1; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)
UniProt Protein Name
Pleiotrophin
Protein Family
UniProt Gene Name
PTN
UniProt Synonym Gene Names
HBNF1; NEGF1; PTN; HBBM; HBGF-8; HB-GAM; HBNF-1; OSF-1
UniProt Entry Name
PTN_HUMAN
Uniprot Description
PTN: Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti- apoptotic signaling of PTN and regulation of cell proliferation. Belongs to the pleiotrophin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 7q33
Cellular Component: extracellular space; endoplasmic reticulum
Molecular Function: heparin binding; growth factor activity; protein phosphatase inhibitor activity
Biological Process: nervous system development; positive regulation of cell division; negative regulation of catalytic activity; positive regulation of cell proliferation; learning; transmembrane receptor protein tyrosine phosphatase signaling pathway; bone mineralization
Research Articles on PTN
1. Over expression of PTN is significantly correlated with poor prognosis in gastric cancer patients.
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Product Notes
The Guinea Pig PTN ptn (Catalog #AAA2510602) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2510602 ELISA Kit recognizes Guinea Pig PTN. It is sometimes possible for the material contained within the vial of "PTN, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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