Rat Corticosteroid Binding Globulin ELISA Kit | CBG elisa kit

Rat CBG (Corticosteroid Binding Globulin) ELISA Kit 

Cat. Num. AAA2533539

• Gene Names: SERPINA6; CBG
• Reactivity: Rat
• Synonyms: Corticosteroid Binding Globulin; Rat CBG (Corticosteroid Binding Globulin) ELISA Kit ; CBG elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes Rat CAT in samples. No significant cross-reactivity or interference between Rat CAT and analogues was observed.
• Sequence Length: 405

• Samples: Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 9.375pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CAT were tested 20 times on one plate, respectively
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CAT were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CBG elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat CAT concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CAT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat CAT. You can calculate the concentration of Rat CAT in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 73858564
• NCBI GeneID: 866
• NCBI Accession #: NP_001747.2
• NCBI GenBank Nucleotide #: NM_001756.3
• Molecular Weight: 45,141 Da
• NCBI Official Full Name: corticosteroid-binding globulin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
• NCBI Official Symbol: SERPINA6
• NCBI Official Synonym Symbols: CBG
• NCBI Protein Information: corticosteroid-binding globulin
• UniProt Protein Name: Corticosteroid-binding globulin
• UniProt Gene Name: SERPINA6
• UniProt Synonym Gene Names: CBG; CBG
• UniProt Entry Name: CBG_HUMAN

NCBI Description

This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPINA6: Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency). CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; steroid binding

Biological Process: glucocorticoid metabolic process; transport

Disease: Corticosteroid-binding Globulin Deficiency