Human Corticosteroid-binding globulin ELISA Kit | SERPINA6 elisa kit

Human Corticosteroid-binding globulin ELISA Kit

Cat. Num. AAA764185

• Gene Names: SERPINA6; CBG
• Reactivity: Human
• Synonyms: Corticosteroid-binding globulin; Human Corticosteroid-binding globulin ELISA Kit; CBG/SERPINA6/Serpin A6/Transcortin/corticosteroid binding globulin/member 6/serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); SERPINA6 elisa kit

• Reactivity: Human
• Sequence Length: 405

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000ng/ml
• Sensitivity: 9.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SERPINA6 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CBG antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CBG antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CBG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CBG can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 73858564
• NCBI GeneID: 866
• NCBI Accession #: NP_001747.2
• NCBI GenBank Nucleotide #: NM_001756.3
• Molecular Weight: 45,141 Da
• NCBI Official Full Name: corticosteroid-binding globulin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
• NCBI Official Symbol: SERPINA6
• NCBI Official Synonym Symbols: CBG
• NCBI Protein Information: corticosteroid-binding globulin
• UniProt Protein Name: Corticosteroid-binding globulin
• Protein Family: Corticosteroid-binding globulin
• UniProt Gene Name: SERPINA6
• UniProt Synonym Gene Names: CBG; CBG
• UniProt Entry Name: CBG_HUMAN

NCBI Description

This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPINA6: Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency). CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; steroid binding

Biological Process: glucocorticoid metabolic process; transport

Disease: Corticosteroid-binding Globulin Deficiency