Human Caveolin-1 ELISA Kit | CAV1 elisa kit

Human Caveolin-1 ELISA Kit

Cat. Num. AAA765437

• Gene Names: CAV1; CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
• Reactivity: Human
• Synonyms: Caveolin-1; Human Caveolin-1 ELISA Kit; Cav-1 (Caveolin-1)/CAV1/BSCL3/CGL3/VIP21/CAV/caveolae protein; 22kD/cell growth-inhibiting protein 32/; CAV1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CAV1. No significant cross-reactivity or interference between CAV1 and analogues was observed.
• Sequence Length: 147

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAV1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 290542363
• NCBI GeneID: 857
• NCBI Accession #: NP_001166368.1
• NCBI GenBank Nucleotide #: NM_001172897.1
• Molecular Weight: 17,023 Da
• NCBI Official Full Name: caveolin-1 isoform beta
• NCBI Official Synonym Full Names: caveolin 1
• NCBI Official Symbol: CAV1
• NCBI Official Synonym Symbols: CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
• NCBI Protein Information: caveolin-1
• UniProt Protein Name: Caveolin-1
• Protein Family: Caveolin
• UniProt Gene Name: CAV1
• UniProt Synonym Gene Names: CAV
• UniProt Entry Name: CAV1_HUMAN

NCBI Description

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]

Uniprot Description

Caveolin-1: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4. Interacts (via the N- terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with BMX and BTK. Expressed in muscle and lung, less so in liver, brain and kidney. Belongs to the caveolin family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q31.1

Cellular Component: acrosomal membrane; apical plasma membrane; basal plasma membrane; basolateral plasma membrane; caveola; cell cortex; cell surface; cilium; cytoplasmic vesicle; cytosol; early endosome membrane; endoplasmic reticulum; endoplasmic reticulum membrane; endosome; focal adhesion; Golgi membrane; integral to plasma membrane; intracellular; lipid particle; lipid raft; membrane; mitochondrion; perinuclear region of cytoplasm; peroxisomal membrane; plasma membrane; protein complex

Molecular Function: ATPase binding; cholesterol binding; enzyme binding; identical protein binding; nitric-oxide synthase binding; patched binding; protease activator activity; protein binding; protein binding, bridging; protein complex scaffold; protein heterodimerization activity; protein kinase binding; Rac GTPase binding; receptor binding; structural molecule activity; syntaxin binding

Biological Process: angiogenesis; blood coagulation; calcium ion homeostasis; calcium ion transport; cellular calcium ion homeostasis; cellular response to starvation; cholesterol efflux; cholesterol homeostasis; cholesterol transport; cytosolic calcium ion homeostasis; inactivation of MAPK activity; lactation; leukocyte migration; maintenance of cellular protein localization; mammary gland development; mammary gland involution; MAPKKK cascade; membrane depolarization; microtubule polymerization; negative regulation of BMP signaling pathway; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of endothelial cell proliferation; negative regulation of epithelial cell differentiation; negative regulation of JAK-STAT cascade; negative regulation of MAPKKK cascade; negative regulation of neuron differentiation; negative regulation of nitric oxide biosynthetic process; negative regulation of nitric-oxide synthase activity; negative regulation of peptidyl-serine phosphorylation; negative regulation of pinocytosis; negative regulation of protein binding; negative regulation of protein ubiquitination; negative regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat5 protein; nitric oxide homeostasis; nitric oxide metabolic process; organ regeneration; positive regulation of endocytosis; positive regulation of endothelial cell proliferation; positive regulation of metalloenzyme activity; positive regulation of microtubule polymerization; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of protein ubiquitination; positive regulation of toll-like receptor 3 signaling pathway; positive regulation of vasoconstriction; protein homooligomerization; protein localization; receptor internalization; receptor mediated endocytosis of virus by host; regulation of blood coagulation; regulation of fatty acid metabolic process; regulation of nitric-oxide synthase activity; regulation of peptidase activity; regulation of smooth muscle contraction; regulation of the force of heart contraction by chemical signal; response to bacterium; response to calcium ion; response to drug; response to estrogen stimulus; response to gamma radiation; response to glucocorticoid stimulus; response to hypoxia; response to nutrient; response to progesterone stimulus; sequestering of lipid; skeletal muscle development; T cell costimulation; triacylglycerol metabolic process; vascular endothelial growth factor receptor signaling pathway; vasculogenesis; vasoconstriction; vesicle organization and biogenesis

Disease: Lipodystrophy, Congenital Generalized, Type 3; Partial Lipodystrophy, Congenital Cataracts, And Neurodegeneration Syndrome; Pulmonary Hypertension, Primary, 3