CAPG recombinant protein

CAPG, 1-348aa, Human, E Coli

Cat. Num. AAA203494

• Gene Names: CAPG; MCP; AFCP; HEL-S-66
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: CAPG; 1-348aa; Human; E Coli; Macrophage-capping protein; MCP; AFCP; Macrophage-capping protein Actin capping protein GCAP39; Actin regulatory protein CAP G; Capping protein (actin filament) gelsolin like; Capping protein gelsolin like; gCap39; Gelsolin like capping protein; Macrophage capping protein; mbh1; Myc basic motif homolog 1.; CAPG recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MYTAIPQSGS PFPGSVQDPG LHVWRVEKLK PVPVAQENQG VFFSGDSYLV LHNGPEEVSH LHLWIGQQSS RDEQGACAVL AVHLNTLLGE RPVQHREVQG NESDLFMSYF PRGLKYQEGG VESAFHKTST GAPAAIKKLY QVKGKKNIRA TERALNWDSF NTGDCFILDL GQNIFAWCGG KSNILERNKA RDLALAIRDS ERQGKAQVEI VTDGEEPAEM IQVLGPKPAL KEGNPEEDLT ADKANAQAAA LYKVSDATGQ MNLTKVADSS PFALELLISD DCFVLDNGLC GKIYIWKGRK ANEKERQAAL QVAEGFISRM QYAPNTQVEI LPQGRESPIF KQFFKDWK
• Sequence Length: 348

• Applicable Applications for CAPG recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CAPG recombinant protein

Macrophage-capping protein, also known as CAPG, belongs to the gelsolin/villin family of actin-regulatory proteins. CAPG is a calcium-sensitive DNA-binding protein that plays a role in macrophage function. It is expressed in macrophages and macrophage-like cells and can localize both to the nucleus and the cytoplasm. This protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. Recombinant human CAPG was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CAPG recombinant protein

Immunology

References

Dabiri G A., et al. (1992) J Biol Chem. 267:16545-16552.; Pellieux C., et al. (2003) J Biol Chem. 278 (3):29136-44.;

NCBI and Uniprot Product Information

• NCBI GI #: 12653873
• NCBI GeneID: 822
• Molecular Weight: 38.5 kDa (348aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: Capping protein (actin filament), gelsolin-like
• NCBI Official Synonym Full Names: capping actin protein, gelsolin like
• NCBI Official Symbol: CAPG
• NCBI Official Synonym Symbols: MCP; AFCP; HEL-S-66
• NCBI Protein Information: macrophage-capping protein
• UniProt Protein Name: Macrophage-capping protein
• Protein Family: Protein
• UniProt Gene Name: CAPG
• UniProt Synonym Gene Names: AFCP; MCP

NCBI Description

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.