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Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate )

Mouse anti-Human CAPG Monoclonal Antibody | anti-CAPG antibody

CAPG antibody

Gene Names
CAPG; MCP; AFCP; HEL-S-66
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
CAPG; Monoclonal Antibody; CAPG antibody; Macrophage-capping protein; MCP; AFCP; Macrophage-capping protein Actin capping protein GCAP39; Actin regulatory protein CAP G; Capping protein (actin filament) gelsolin like; Capping protein gelsolin like; gCap39; Gelsolin like capping protein; Macrophage capping protein; mbh1; Myc basic motif homolog 1.; anti-CAPG antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1D10
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
348
Applicable Applications for anti-CAPG antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human CAPG (1-348aa) purified from E. coli
Clone Note
Anti-human CAPG mAb, clone AT1D10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CAPG protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate )

Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate )

Immunofluorescence (IF)

(ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Immunofluorescence (IF) (ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-CAPG antibody
Macrophage-capping protein, also known as CAPG, belongs to the gelsolin/villin family of actin-regulatory proteins. CAPG is a calcium-sensitive DNA-binding protein that plays a role in macrophage function. It is expressed in macrophages and macrophage-like cells and can localize both to the nucleus and the cytoplasm. This protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments.
Product Categories/Family for anti-CAPG antibody
References
Kumar A., et al. (2015) Mol Cell Endocrinol. 404: 91-101.; Dabiri G A., et al. (1992) J Biol Chem. 267: 16545-16552.;

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
822
Molecular Weight
36,857 Da
NCBI Official Full Name
Capping protein (actin filament), gelsolin-like
NCBI Official Synonym Full Names
capping actin protein, gelsolin like
NCBI Official Symbol
CAPG
NCBI Official Synonym Symbols
MCP; AFCP; HEL-S-66
NCBI Protein Information
macrophage-capping protein
UniProt Protein Name
Macrophage-capping protein
Protein Family
UniProt Gene Name
CAPG
UniProt Synonym Gene Names
AFCP; MCP

NCBI Description

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.

Research Articles on CAPG

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Product Notes

The CAPG capg (Catalog #AAA200419) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CAPG antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CAPG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the CAPG capg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAPG, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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