Canine Stem Cell Factor (SCF) ELISA Kit | SCF elisa kit

Canine Stem Cell Factor (SCF) ELISA Kit

Cat. Num. AAA452682

• Gene Names: KITLG; CSF; MGF; 710-712
• Reactivity: Canine
• Synonyms: Stem Cell Factor (SCF); Canine Stem Cell Factor (SCF) ELISA Kit; KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2; SCF elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of SCF. No significant cross-reactivity or interference between SCF and analogues was observed.
• Sequence Length: 274

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 6.5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SCF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SCF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCF elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SCF in canine serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SCF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SCF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SCF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SCF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 60678250
• NCBI GeneID: 403507
• NCBI Accession #: NP_001012753.1
• NCBI GenBank Nucleotide #: NM_001012735.2
• UniProt Accession #: Q06220; Q8SPM6
• Molecular Weight: 30,870 Da
• NCBI Official Full Name: kit ligand
• NCBI Official Symbol: KITLG
• NCBI Official Synonym Symbols: CSF; MGF; 710-712
• NCBI Protein Information: kit ligand
• UniProt Protein Name: Kit ligand
• UniProt Gene Name: KITLG
• UniProt Synonym Gene Names: MGF; MGF; SCF; sKITLG

Uniprot Description

Stimulates the proliferation of mast cells. Able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Mediates also cell-cell adhesion. Acts synergistically with other cytokines, probably interleukins.