Mouse MEF2C Monoclonal Antibody | anti-MEF2C antibody

MEF2C antibody

Cat. Num. AAA833917

• Gene Names: MEF2C; DEL5q14.3; C5DELq14.3
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: MEF2C antibody was purified by affinity chromatography.
• Synonyms: MEF2C; Monoclonal Antibody; MEF2C antibody; Monoclonal MEF2C; Anti-MEF2C; Myocyte enhancer factor 2C; OTTHUMP00000222410; MADS box transcription enhancer factor 2 polypeptide C; Myocyte specific enhancer factor 2C; DEL5q14.3; MEFC 2; MEFC-2; anti-MEF2C antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4B10
• Purity/Purification: MEF2C antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 473

• Applicable Applications for anti-MEF2C antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 1:100; FC: 1:100; WB: 1:500
• Biological Significance: This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation.
• Cross-Reactivity: Human
• Immunogen: MEF2C antibody was raised in mouse using a full length recombinant protein of human MEF2C (NP_002388) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using MEF2C antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant MEF2C protein (Right) or empty vector (Left) detected with MEF2C antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant MEF2C protein using MEF2C antibody)

Related Product Information for anti-MEF2C antibody

Mouse monoclonal MEF2C antibody

Product Categories/Family for anti-MEF2C antibody

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 19923215
• NCBI GeneID: 4208
• NCBI Accession #: NP_002388
• NCBI GenBank Nucleotide #: NM_002397.4
• UniProt Accession #: Q06413; C9JMZ0; D7F7N5; F8W7V7
• Molecular Weight: 50,242 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2C isoform 1
• NCBI Official Synonym Full Names: myocyte enhancer factor 2C
• NCBI Official Symbol: MEF2C
• NCBI Official Synonym Symbols: DEL5q14.3; C5DELq14.3
• NCBI Protein Information: myocyte-specific enhancer factor 2C
• UniProt Protein Name: Myocyte-specific enhancer factor 2C
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2C
• UniProt Entry Name: MEF2C_HUMAN

NCBI Description

This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010]

Uniprot Description

MEF2C: transcription factor of the MADS family which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. May be involved in myogenesis, neurogenesis and in the development of cortical architecture. Three splice-variant isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 5q14.3

Cellular Component: nucleoplasm; protein complex; intracellular membrane-bound organelle; cytoplasm; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; miRNA binding; transcription activator binding; DNA binding; AT DNA binding; protein heterodimerization activity; histone deacetylase binding; chromatin binding; transcription factor activity

Biological Process: cardiac muscle hypertrophy; muscle development; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; heart development; regulation of neurotransmitter secretion; stress-activated MAPK cascade; regulation of synaptic plasticity; toll-like receptor 3 signaling pathway; toll-like receptor 5 signaling pathway; neuron differentiation; positive regulation of MAP kinase activity; B cell receptor signaling pathway; regulation of neuron apoptosis; negative regulation of ossification; positive regulation of B cell proliferation; chondrocyte differentiation; negative regulation of neuron apoptosis; toll-like receptor 4 signaling pathway; positive regulation of cardiac muscle cell proliferation; endochondral ossification; nervous system development; skeletal muscle development; monocyte differentiation; response to virus; myotube differentiation; positive regulation of skeletal muscle development; muscle cell fate determination; toll-like receptor 2 signaling pathway; muscle cell differentiation; regulation of synaptogenesis; neuron development; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; B cell proliferation; transcription from RNA polymerase II promoter; regulation of synaptic transmission, glutamatergic; apoptosis; neuron migration; ventricular cardiac muscle cell differentiation; regulation of synaptic activity; negative regulation of transcription from RNA polymerase II promoter; palate development; toll-like receptor 10 signaling pathway; learning and/or memory; regulation of transcription, DNA-dependent; B cell homeostasis; regulation of megakaryocyte differentiation; melanocyte differentiation; germinal center formation; neural crest cell differentiation; heart looping; negative regulation of epithelial cell proliferation; blood vessel development; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; embryonic viscerocranium morphogenesis; positive regulation of bone mineralization; regulation of germinal center formation; positive regulation of myoblast differentiation; humoral immune response; osteoblast differentiation; MyD88-dependent toll-like receptor signaling pathway; positive regulation of osteoblast differentiation; neuron morphogenesis during differentiation; toll-like receptor signaling pathway; positive regulation of muscle cell differentiation; innate immune response; blood vessel remodeling; platelet formation; positive regulation of neuron differentiation; regulation of excitatory postsynaptic membrane potential

Disease: Mental Retardation, Autosomal Dominant 20