Western Blot (WB)
(AKR1E2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the AKR1E2 antibody detected the AKR1E2 protein (arrow).)
Rabbit anti-Human AKR1E2 Polyclonal Antibody | anti-AKR1E2 antibody
AKR1E2, CT (AKR1E2, AKR1CL2, AKRDC1, 1,5-anhydro-D-fructose reductase, Aldo-keto reductase family 1 member C-like protein 2, Aldo-keto reductase family 1 member E2, LoopADR, Testis-specific protein) (FITC)
Gene Names
AKR1E2; hTSP; htAKR; AKRDC1; AKR1CL2; LoopADR
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
AKR1E2; Polyclonal Antibody; CT (AKR1E2; AKR1CL2; AKRDC1; 1; 5-anhydro-D-fructose reductase; Aldo-keto reductase family 1 member C-like protein 2; Aldo-keto reductase family 1 member E2; LoopADR; Testis-specific protein) (FITC); Testis-specific protein; anti-AKR1E2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-AKR1E2 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
AKR1E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 290-320 amino acids selected from the C-terminal region of human AKR1E2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(AKR1E2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the AKR1E2 antibody detected the AKR1E2 protein (arrow).)
Western Blot (WB)
(AKR1E2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the AKR1E2 antibody detected the AKR1E2 protein (arrow).)
Related Product Information for anti-AKR1E2 antibody
AKR1E2 catalyzes the NADPH-dependent reduction of 1,5-anhydro-D-fructose (AF) to 1,5-anhydro-D-glucitol. Can also catalyze the reduction of various aldehydes and quinones (By similarity). Has low NADPH-dependent reductase activity towards 9,10-phenanthrenequinone (in vitro).
Product Categories/Family for anti-AKR1E2 antibody
References
Clancy, R.M., et al. Arthritis Rheum. 62(11):3415-3424(2010). Lamesch, P., et al. Genomics 89(3):307-315(2007). Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Azuma, Y., et al. Mol. Hum. Reprod. 10(7):527-533(2004). Nishinaka, T., et al. Chem. Biol. Interact. 143-144, 299-305 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,589 Da
NCBI Official Full Name
1,5-anhydro-D-fructose reductase isoform 1
NCBI Official Synonym Full Names
aldo-keto reductase family 1, member E2
NCBI Official Symbol
AKR1E2
NCBI Official Synonym Symbols
hTSP; htAKR; AKRDC1; AKR1CL2; LoopADR
NCBI Protein Information
1,5-anhydro-D-fructose reductase; AF reductase; testis-specific protein; aldo-keto reductase loopADR; aldo-keto reductase family 1, member C-like 2; aldo-keto reductase family 1 member C-like protein 2
UniProt Protein Name
1,5-anhydro-D-fructose reductase
Protein Family
UniProt Gene Name
AKR1E2
UniProt Synonym Gene Names
AKR1CL2; AKRDC1; AF reductase; hTSP
UniProt Entry Name
AKCL2_HUMAN
NCBI Description
The protein encoded by this gene is a member of the aldo-keto reductase superfamily. Members in this family are characterized by their structure (evolutionarily highly conserved TIM barrel) and function (NAD(P)H-dependent oxido-reduction of carbonyl groups). Transcripts of this gene have been reported in specimens of human testis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
AKR1CL2: Catalyzes the NADPH-dependent reduction of 1,5-anhydro- D-fructose (AF) to 1,5-anhydro-D-glucitol. Can also catalyze the reduction of various aldehydes and quinones. Has low NADPH-dependent reductase activity towards 9,10- phenanthrenequinone (in vitro). Belongs to the aldo/keto reductase family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; EC 1.1.1.263
Chromosomal Location of Human Ortholog: 10p15.1
Cellular Component: cytoplasm
Molecular Function: 1,5-anhydro-D-fructose reductase activity
Research Articles on AKR1E2
Similar Products
Product Notes
The AKR1E2 akr1e2 (Catalog #AAA6272589) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKR1E2, CT (AKR1E2, AKR1CL2, AKRDC1, 1,5-anhydro-D-fructose reductase, Aldo-keto reductase family 1 member C-like protein 2, Aldo-keto reductase family 1 member E2, LoopADR, Testis-specific protein) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AKR1E2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AKR1E2 akr1e2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKR1E2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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