Typical Testing Data/Standard Curve (for reference only)
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Canine Interleukin 34, IL-34 ELISA Kit | IL-34 elisa kit
Canine Interleukin 34, IL-34 ELISA Kit
Gene Names
IL34; IL-34; C16orf77
Reactivity
Canine
Synonyms
Interleukin 34; IL-34; Canine Interleukin 34; IL-34 ELISA Kit; IL-34 elisa kit
Reactivity
Canine
Sequence Length
242
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.5-150ng/L
Sensitivity
0.24ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for IL-34 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Canine Interleukin 34 (also known as IL-34) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine IL-34 antibody. IL-34 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine IL-34 Antibody is added and binds to IL-34 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-34 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine IL-34. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine IL-34 antibody. IL-34 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine IL-34 Antibody is added and binds to IL-34 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-34 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine IL-34. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,354 Da
NCBI Official Full Name
interleukin-34 isoform 1
NCBI Official Synonym Full Names
interleukin 34
NCBI Official Symbol
IL34
NCBI Official Synonym Symbols
IL-34; C16orf77
NCBI Protein Information
interleukin-34
UniProt Protein Name
Interleukin-34
UniProt Gene Name
IL34
UniProt Synonym Gene Names
C16orf77; IL-34
NCBI Description
Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008]
Uniprot Description
Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
Research Articles on IL-34
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Product Notes
The Canine IL-34 il34 (Catalog #AAA1602630) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1602630 ELISA Kit recognizes Canine IL-34. It is sometimes possible for the material contained within the vial of "Interleukin 34, IL-34, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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