Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine IL13 antibody. IL13 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine IL13 Antibody is added and binds to IL13 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL13 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine IL13. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI Description
cytokine; involved in inflammatory and immune responses [RGD, Feb 2006]
Uniprot Description
IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane
Molecular Function: interleukin-13 receptor binding; cytokine activity
Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; regulation of proton transport; microglial cell activation; response to lipopolysaccharide; positive regulation of mast cell degranulation; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; response to ethanol; positive regulation of protein secretion; response to mechanical stimulus; positive regulation of B cell proliferation; positive regulation of ion transport; immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of NAD(P)H oxidase activity; inflammatory response; positive regulation of macrophage activation
Research Articles on IL13
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Product Notes
The Canine IL13 il13 (Catalog #AAA1604383) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1604383 ELISA Kit recognizes Canine IL13. It is sometimes possible for the material contained within the vial of "Interleukin-13, IL13, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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