Rabbit anti-Human WDR51B Polyclonal Antibody | anti-WDR51B antibody

WDR51B, CT (POC1B, WDR51B, POC1 centriolar protein homolog B, WD repeat-containing protein 51B) (PE)

Cat. Num. AAA6363345

• Gene Names: POC1B; PIX1; CORD20; TUWD12; WDR51B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WDR51B; Polyclonal Antibody; CT (POC1B; POC1 centriolar protein homolog B; WD repeat-containing protein 51B) (PE); POC1B; WD repeat-containing protein 51B; anti-WDR51B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 478

• Applicable Applications for anti-WDR51B antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: WDR51B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 321-350 amino acids from the C-terminal region of human WDR51B.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of WDR51B Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in MDA-MB453 cell line lysate. WDR51B Antibody (C-term) (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(WDR51B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR51B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-WDR51B antibody

Antibodies; WD Repeat Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Pearson, C.G., et al. J. Cell Biol. 187(6):905-920(2009). Keller, L.C., et al. Mol. Biol. Cell 20(4):1150-1166(2009). Hames, R.S., et al. Exp. Cell Res. 314(3):574-589(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 26665869
• NCBI GeneID: 282809
• NCBI Accession #: NP_758440.1
• NCBI GenBank Nucleotide #: NP_758440.1
• UniProt Accession #: Q8TC44
• NCBI Official Full Name: POC1 centriolar protein homolog B isoform a
• NCBI Official Synonym Full Names: POC1 centriolar protein B
• NCBI Official Symbol: POC1B
• NCBI Official Synonym Symbols: PIX1; CORD20; TUWD12; WDR51B
• NCBI Protein Information: POC1 centriolar protein homolog B
• UniProt Protein Name: POC1 centriolar protein homolog B
• UniProt Gene Name: POC1B
• UniProt Synonym Gene Names: WDR51B

NCBI Description

POC1 proteins contain an N-terminal WD40 domain and a C-terminal coiled coil domain and are part of centrosomes. They play an important role in basal body and cilia formation. This gene encodes one of the two POC1 proteins found in humans. Mutation in this gene result in autosomal-recessive cone-rod dystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

WDR51B: Required for ciliogenesis. Belongs to the WD repeat POC1 family.

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: centriole; centrosome; spindle pole

Molecular Function: protein binding

Biological Process: cell proliferation; retinal homeostasis

Disease: Cone-rod Dystrophy 20