Human calbindin 1, 28kDa ELISA Kit | CALB1 elisa kit

Human Calbindin, CALB1 ELISA Kit

Cat. Num. AAA928501

• Gene Names: CALB1; CALB
• Reactivity: Human
• Synonyms: calbindin 1; 28kDa; Human Calbindin; CALB1 ELISA Kit; Human Calbindin (CALB1) ELISA kit; CALB; RTVL-H protein; (28kD) ; CALB1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CALB1. No significant cross-reactivity or interference between Human CALB1 and analogues was observed.
• Sequence Length: 261

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CALB1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for calbindin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any calbindin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for calbindin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of calbindin bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4826655
• NCBI GeneID: 793
• NCBI Accession #: NP_004920.1
• NCBI GenBank Nucleotide #: NM_004929.2
• UniProt Accession #: P05937; B2R696; B7Z9J4
• Molecular Weight: 30,025 Da
• NCBI Official Full Name: calbindin
• NCBI Official Synonym Full Names: calbindin 1, 28kDa
• NCBI Official Symbol: CALB1
• NCBI Official Synonym Symbols: CALB
• NCBI Protein Information: calbindin; D-28K; calbindin D28; RTVL-H protein; calbindin 1, (28kD); vitamin D-dependent calcium-binding protein, avian-type
• UniProt Protein Name: Calbindin
• Protein Family: Calbindin
• UniProt Gene Name: CALB1
• UniProt Synonym Gene Names: CAB27
• UniProt Entry Name: CALB1_HUMAN

NCBI Description

Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq, Jul 2008]

Uniprot Description

CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: cell soma; axon; dendrite; synapse; cytosol; nucleus

Molecular Function: vitamin D binding; protein binding; zinc ion binding; calcium ion binding

Biological Process: cytosolic calcium ion homeostasis; long-term memory; short-term memory; regulation of synaptic plasticity; locomotory behavior