SynCam / CADM1 / TSLC1 / IGSF4 recombinant protein

Recombinant Human SynCam / CADM1 / TSLC1 / IGSF4 Protein (His tag)

Cat. Num. AAA2545887

• Gene Names: CADM1; BL2; ST17; IGSF4; NECL2; RA175; TSLC1; IGSF4A; Necl-2; SYNCAM; sgIGSF; sTSLC-1; synCAM1
• Purity: > 94 % as determined by SDS-PAGE
• Synonyms: SynCam / CADM1 / TSLC1 / IGSF4; Recombinant Human SynCam / CADM1 / TSLC1 / IGSF4 Protein (His tag); BL2; DKFZp686F1789; IGSF4; IGSF4A; MGC149785; MGC51880; NECL2; Necl-2; RA175; ST17; SYNCAM; TSLC1; sTSLC-1; sgIGSF; synCAM1; SynCam / CADM1 / TSLC1 / IGSF4 recombinant protein

• Host: Human Cells
• Purity/Purification: > 94 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 442

• Application Notes: The secreted recombinant human CADM1 consists of 341 amino acids after removal of the signal peptide and has a predicted molecular mass of 38.5 kDa. As a result of glycosylation, the apparent molecular masss of rh CADM1 is approximately 70-80 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 45
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for SynCam / CADM1 / TSLC1 / IGSF4 recombinant protein

Background: Members of the immunoglobulin superfamily often play key roles in intercellular adhesion. IGSF4 is a novel immunoglobulin (Ig)-like intercellular adhesion molecule. Three Ig-like domains are included in the extracellular domain of IGSF4 and mediate homophilic or heterophilic interactions independently of Ca2+. The cytoplasmic domain of IGSF4 contains the binding motifs that connect to actin fibers. Since IGSF4 has been characterized by several independent research groups, this molecule is called by three names, TSLC1, SgIGSF and SynCAM. IGSF4 was first characterized as a tumor suppressor of non-small cell lung cancer and termed TSLC1. It is a single-pass type I membrane protein which belongs to the nectin family, which may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. In addition, CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. In neuroblastoma, loss of CADM1 expression has recently been found in disseminated tumours with adverse outcome, prompting us to investigate its role in neuroblastoma tumour progression. The downregulation of CADM1 tumour suppressor gene expression is a critical event in neuroblastoma pathogenesis resulting in tumour progression.

Description: A DNA sequence encoding the human CADM1 (NP_055148.3) extracellular domain (Met 1-His 374) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 148664190
• NCBI GeneID: 23705
• NCBI Accession #: NP_055148.3
• NCBI GenBank Nucleotide #: NM_014333.3
• UniProt Accession #: Q9BY67; Q86WB8; Q8N2F4; A4FVB5; F5H0J4; H0YGA7; H1ZZV9; H1ZZW1; H1ZZW2; X5D2C8
• Molecular Weight: 45,624 Da
• NCBI Official Full Name: cell adhesion molecule 1 isoform D
• NCBI Official Synonym Full Names: cell adhesion molecule 1
• NCBI Official Symbol: CADM1
• NCBI Official Synonym Symbols: BL2; ST17; IGSF4; NECL2; RA175; TSLC1; IGSF4A; Necl-2; SYNCAM; sgIGSF; sTSLC-1; synCAM1
• NCBI Protein Information: cell adhesion molecule 1
• UniProt Protein Name: Cell adhesion molecule 1
• UniProt Gene Name: CADM1
• UniProt Synonym Gene Names: IgSF4; NECL-2; SgIgSF; SynCAM; TSLC-1

Uniprot Description

CADM1: a single-pass type I membrane protein that ediates homophilic cell-cell adhesion, and heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell adhesion; Membrane protein, integral; Tumor suppressor; Vesicle

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: basolateral plasma membrane; cell-cell adherens junction; integral to plasma membrane; intercellular junction; plasma membrane

Molecular Function: cell adhesion molecule binding; PDZ domain binding; protein binding; protein homodimerization activity; receptor activity; receptor binding

Biological Process: activated T cell proliferation; cell recognition; detection of stimulus; heterophilic cell adhesion; homophilic cell adhesion; positive regulation of cytokine secretion; positive regulation of natural killer cell mediated cytotoxicity; susceptibility to natural killer cell mediated cytotoxicity; T cell mediated cytotoxicity