CADM1 active protein

CADM1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120713

• Gene Names: CADM1; BL2; ST17; IGSF4; NECL2; RA175; TSLC1; IGSF4A; Necl-2; SYNCAM; sgIGSF; sTSLC-1; synCAM1
• Purity: >94% as determined by SDS-PAGE
• Synonyms: CADM1; CADM1 Protein; Human; Recombinant (His Tag); Human SynCam/CADM1/TSLC1/IGSF4 Protein (His Tag); BL2 Protein; IGSF4 Protein; IGSF4A Protein; Necl-2 Protein; NECL2 Protein; RA175 Protein; sgIGSF Protein; ST17 Protein; sTSLC-1 Protein; SYNCAM Protein; synCAM1 Protein; TSLC1 Protein; cell adhesion molecule 1; CADM1 active protein

• Host: HEK293 Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His374

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human CADM1 at 2ug/ml (100uL/well) can bind biotinylated human CRTAM with a linear range of 12.5-400ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 45
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CADM1 (NP_055148.3) extracellular domain (Met 1-His 374) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CADM1 active protein

Background: Members of the immunoglobulin superfamily often play key roles in intercellular adhesion. IGSF4 is a novel immunoglobulin (Ig)-like intercellular adhesion molecule. Three Ig-like domains are included in the extracellular domain of IGSF4 and mediate homophilic or heterophilic interactions independently of Ca2+. The cytoplasmic domain of IGSF4 contains the binding motifs that connect to actin fibers. Since IGSF4 has been characterized by several independent research groups, this molecule is called by three names, TSLC1, SgIGSF and SynCAM. IGSF4 was first characterized as a tumor suppressor of non-small cell lung cancer and termed TSLC1. It is a single-pass type I membrane protein which belongs to the nectin family, which may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. In addition, CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. In neuroblastoma, loss of CADM1 expression has recently been found in disseminated tumours with adverse outcome, prompting us to investigate its role in neuroblastoma tumour progression. The downregulation of CADM1 tumour suppressor gene expression is a critical event in neuroblastoma pathogenesis resulting in tumour progression.

References

Watabe K, et al. (2003) IGSF4: a new intercellular adhesion molecule that is called by three names, TSLC1, SgIGSF and SynCAM, by virtue of its diverse function. Histol Histopathol. 18(4): 1321-9.Fujita E, et al. (2005) Distribution of RA175/TSLC1/SynCAM, a member of the immunoglobulin superfamily, in the developing nervous system. Brain Res Dev Brain Res. 154(2): 199-209.Fujita E, et al. (2006) Oligo-astheno-teratozoospermia in mice lacking RA175/TSLC1/SynCAM/IGSF4A, a cell adhesion molecule in the immunoglobulin superfamily. Mol Cell Biol. 26(2): 718-26.Nowacki S, et al. (2008) Expression of the tumour suppressor gene CADM1 is associated with favourable outcome and inhibits cell survival in neuroblastoma. Oncogene. 27(23): 3329-38.

NCBI and Uniprot Product Information

• NCBI GI #: 148664190
• NCBI GeneID: 23705
• NCBI Accession #: NP_055148.3
• NCBI GenBank Nucleotide #: NM_014333.3
• UniProt Accession #: Q9BY67; Q86WB8; Q8N2F4; A4FVB5; F5H0J4; H0YGA7; H1ZZV9; H1ZZW1; H1ZZW2
• Molecular Weight: 48,509 Da
• NCBI Official Full Name: cell adhesion molecule 1 isoform D
• NCBI Official Synonym Full Names: cell adhesion molecule 1
• NCBI Official Symbol: CADM1
• NCBI Official Synonym Symbols: BL2; ST17; IGSF4; NECL2; RA175; TSLC1; IGSF4A; Necl-2; SYNCAM; sgIGSF; sTSLC-1; synCAM1
• NCBI Protein Information: cell adhesion molecule 1; TSLC-1; nectin-like 2; nectin-like protein 2; truncated CADM1 protein; TSLC1/Nectin-like 2/IGSF4; synaptic cell adhesion molecule; tumor suppressor in lung cancer 1; immunoglobulin superfamily member 4; immunoglobulin superfamily
• UniProt Protein Name: Cell adhesion molecule 1
• Protein Family: Cell adhesion molecule
• UniProt Gene Name: CADM1
• UniProt Synonym Gene Names: IGSF4; IGSF4A; NECL2; SYNCAM; TSLC1; IgSF4; NECL-2; SgIgSF; SynCAM; TSLC-1
• UniProt Entry Name: CADM1_HUMAN

Uniprot Description

CADM1: a single-pass type I membrane protein that ediates homophilic cell-cell adhesion, and heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Vesicle; Apoptosis; Tumor suppressor; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.2

Cellular Component: synaptic vesicle; basolateral plasma membrane; axon; dendrite; integral to membrane; plasma membrane; intercellular junction

Molecular Function: protein binding; protein homodimerization activity; cell adhesion molecule binding; receptor binding; PDZ domain binding

Biological Process: unidimensional cell growth; intercellular junction assembly and maintenance; apoptosis; activated T cell proliferation; cell recognition; susceptibility to natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity; liver development; calcium-independent cell-cell adhesion; heterophilic cell adhesion; synaptogenesis; detection of stimulus; spermatogenesis; brain development; homophilic cell adhesion; cell differentiation; T cell mediated cytotoxicity; positive regulation of cytokine secretion