Western Blot (WB)
(Anti-Adrb3 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:44 kDa Observed band size:35 kDa)
Rabbit anti-Rat ADRB3 Polyclonal Antibody | anti-ADRB3 antibody
Anti-ADRB3 Antibody, Rabbit Polyclonal
Gene Names
Adrb3; ADRB
Reactivity
Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
ADRB3; Polyclonal Antibody; Anti-ADRB3 Antibody; Rabbit Polyclonal; ADRB3 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-ADRB3 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rat ADRB3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ADRB3 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IP: 0.2-1uL/mg of lysate
IP: 0.2-1uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the C-terminus of the Rat ADRB3
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Rat ADRB3, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-Adrb3 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:44 kDa Observed band size:35 kDa)
Western Blot (WB)
(Anti-Adrb3 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:44 kDa Observed band size:35 kDa)
Immunoprecipitation (IP)
(Adrb3 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 1 uL anti-Adrb3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Adrb3 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 45 kDa Observed band size: 35 kDa)
Immunoprecipitation (IP)
(Adrb3 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate 1 uL anti-Adrb3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Adrb3 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 45 kDa Observed band size: 35 kDa)
Related Product Information for anti-ADRB3 antibody
beta3-Adrenergic receptor (ADRB3) Trp64Arg polymorphism is associated with atherogenic risk factors that include weight gain, insulin resistance, and diabetes. Habitual exercise brings higher cardiorespiratory fitness and results in the amelioration of atherosclerotic risk factors. Presence of the beta3-Adrenergic receptor (ADRB3) gene Trp64Arg (T64A) polymorphism may be associated with an increased susceptibility for essential hypertension (EH). The beta3-adrenergic receptor (ADRB3) regulates thermogenesis and lipolysis in brown and white adipose tissue. ADRB3 plays a critical role in the regulation of energy metabolism in mammals. Polymorphism in the ovine ADRB3 gene has been reported to be associated with birth weight, growth rate, carcass composition, and cold survival.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,146 Da
NCBI Official Full Name
beta-3 adrenergic receptor
NCBI Official Synonym Full Names
adrenoceptor beta 3
NCBI Official Symbol
Adrb3
NCBI Official Synonym Symbols
ADRB
NCBI Protein Information
beta-3 adrenergic receptor
UniProt Protein Name
Beta-3 adrenergic receptor
Protein Family
UniProt Gene Name
Adrb3
UniProt Synonym Gene Names
Adrb3r; Beta-3 adrenoceptor
NCBI Description
binds BRL373 and norepenephrine; may play a role in the adrenergic signaling pathway [RGD, Feb 2006]
Uniprot Description
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
Research Articles on ADRB3
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Product Notes
The ADRB3 adrb3 (Catalog #AAA8102424) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ADRB3 Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADRB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the ADRB3 adrb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADRB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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