CABP5 recombinant protein

CABP5 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121566

• Gene Names: CABP5; CABP3
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CABP5; CABP5 Protein; Human; Recombinant (His Tag); Human CABP3/CABP5 Protein (His Tag); CABP3 Protein; calcium binding protein 5; CABP5 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 0.1% Tween 20, 50mM Arg, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg173

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human CABP5 (Q9NP86)(Met1-Arg173) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CABP5 recombinant protein

Background: CABP3, also known as CABP5, belongs to a subfamily of calcium binding proteins, which share similarity to calmodulin. Calcium binding proteins are an important component of calcium mediated cellular signal transduction. Expression of CABP3 gene is retina-specific. The mouse homolog of CABP3 has been shown to express in the inner nuclear layer of the retina, suggested its role in neuronal functioning. The specific function of CABP3 gene is unknown. Study of the transcripts and genomic structure revealed that the 5 end of this gene is complementary and reverse to that of the CABP5 gene, and the sequence beyond the overlapping region is nearly identical to that of CABP5. Thus, these two genes encode the protein products with distinct N-terminal halves but identical C-terminal halves. CABP3 inhibits calcium-dependent inactivation of L-type calcium channel and shifts voltage dependence of activation to more depolarized membrane potentials. It is also involved in the transmission of light signals.

References

McCauliffe DP, et al. (1990) A human Ro/SS-A autoantigen is the homologue of calreticulin and is highly homologous with onchocercal RAL-1 antigen and an aplysia memory molecule. J Clin Invest. 86(1):332-5.Beutler E, et al. (1997) HLA-H and associated proteins in patients with hemochromatosis. Mol Med. 3(6):397-402.Michalak M, et al. (2002) Calreticulin in cardiac development and pathology. Biochim Biophys Acta. 1600(1-2):32-7.

NCBI and Uniprot Product Information

• NCBI GI #: 11067753
• NCBI GeneID: 56344
• NCBI Accession #: NP_062829.1
• NCBI GenBank Nucleotide #: NM_019855.4
• UniProt Accession #: Q9NP86; A0AUY4
• Molecular Weight: 19,826 Da
• NCBI Official Full Name: calcium-binding protein 5
• NCBI Official Synonym Full Names: calcium binding protein 5
• NCBI Official Symbol: CABP5
• NCBI Official Synonym Symbols: CABP3
• NCBI Protein Information: calcium-binding protein 5; calcium-binding protein 3
• UniProt Protein Name: Calcium-binding protein 5
• Protein Family: Calcium-binding protein
• UniProt Gene Name: CABP5
• UniProt Synonym Gene Names: CABP3; CaBP5
• UniProt Entry Name: CABP5_HUMAN

NCBI Description

The product of this gene belongs to a subfamily of calcium binding proteins, which share similarity to calmodulin. Calcium binding proteins are an important component of calcium mediated cellular signal transduction. Expression of this gene is retina-specific. The mouse homolog of this protein has been shown to express in the inner nuclear layer of the retina, suggested its role in neuronal functioning. The specific function of this gene is unknown. [provided by RefSeq, Oct 2009]

Uniprot Description

CABP5: Inhibits calcium-dependent inactivation of L-type calcium channel and shifts voltage dependence of activation to more depolarized membrane potentials. Involved in the transmission of light signals. Interacts with CACNA1C (via C-terminal CDB motif) in a calcium-dependent manner

Protein type: Cytoskeletal; Calcium-binding

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cytosol

Molecular Function: calcium ion binding

Biological Process: signal transduction