SDS-Page
C8G recombinant protein
C8G, 21-202aa, Human, His tag, E Coli
Gene Names
C8G; C8C
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
C8G; 21-202aa; Human; His tag; E Coli; complement component 8 gamma polypeptide; complement component 8; gamma polypeptide; C8C; C8G recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.
Concentration
0.5 mg/ml (determined by Bradford) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MQKPQRPRRP ASPISTIQPK ANFDAQQFAG TWLLVAVGSA CRFLQEQGHR AEATTLHVAP QGTAMAVSTF RKLDGICWQV RQLYGDTGVL GRFLLQARGA RGAVHVVVAE TDYQSFAVLY LERAGQLSVK LYARSLPVSD SVLSGFEQRV QEAHLTEDQI FYFPKYGFCE AADQFHVLDE VRR
Sequence Length
202
Applicable Applications for C8G recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for C8G recombinant protein
C8gamma, also known as C8G, is one of three polypeptides (along with C8 alpha and C8 beta) that constitutes C8, a component of the complement system. C8 participates in the formation of Membrane Attack Complex (MAC). Patients with deficiency in C8 are vulnerable to certain bacteria infection. C8gamma is a secreted protein that is able to bind retinol and belongs to the lipocalin family and calycin superfamily. It is synthesised in the liver, monocytes and fibroblast and functions to clear pathogens from an infected host. Recombinant human C8G protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for C8G recombinant protein
References
Chiswell B., et al. (2007) Biochim Biophys Acta. 1774 (5):637-44.; Schreck SF., et al. (2000) Biochim Biophys Acta. 1482 (1-2):199-208.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22.6 kDa (203aa), confirmed by MALDI-TOF
NCBI Official Full Name
Complement component 8, gamma polypeptide
NCBI Official Synonym Full Names
complement C8 gamma chain
NCBI Official Symbol
C8G
NCBI Official Synonym Symbols
C8C
NCBI Protein Information
complement component C8 gamma chain
UniProt Protein Name
Complement component C8 gamma chain
Protein Family
UniProt Gene Name
C8G
NCBI Description
The protein encoded by this gene belongs to the lipocalin family. It is one of the three subunits that constitutes complement component 8 (C8), which is composed of a disulfide-linked C8 alpha-gamma heterodimer and a non-covalently associated C8 beta chain. C8 participates in the formation of the membrane attack complex (MAC) on bacterial cell membranes. While subunits alpha and beta play a role in complement-mediated bacterial killing, the gamma subunit is not required for the bactericidal activity. [provided by RefSeq, Jul 2011]
Uniprot Description
C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol.
Research Articles on C8G
Similar Products
Product Notes
The C8G c8g (Catalog #AAA204109) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's C8G can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the C8G c8g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MQKPQRPRRP ASPISTIQPK ANFDAQQFAG TWLLVAVGSA CRFLQEQGHR AEATTLHVAP QGTAMAVSTF RKLDGICWQV RQLYGDTGVL GRFLLQARGA RGAVHVVVAE TDYQSFAVLY LERAGQLSVK LYARSLPVSD SVLSGFEQRV QEAHLTEDQI FYFPKYGFCE AADQFHVLDE VRR. It is sometimes possible for the material contained within the vial of "C8G, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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