C1QB / C1qB recombinant protein

Recombinant Human C1QB / C1qB Protein (His tag)

Cat. Num. AAA2546002

• Purity: > 94 % as determined by SDS-PAGE
• Synonyms: C1QB / C1qB; Recombinant Human C1QB / C1qB Protein (His tag); C1QB; C1QB / C1qB recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 94 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM TCEP, 10% glycerol, pH 7.4
• Sequence Length: 253

• Application Notes: The recombinant human C1QB consists of 237 amino acids with the predicted molecular mass of 25 kDa. As a result of glycosylation, rhC1QB migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for C1QB / C1qB recombinant protein

Background: Complement Component 1, q subcomponent (C1q) associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Southern blot analysis of chromosomal DNA from vertebrate species demonstrated highest similarity between the C1qB genes, followed by C1qC and finally C1qA. Sequence comparison of C1q from three different species have shown that the B chains have the strongest similarity. C1q was already present at embryonic day 14 (E14) and showed little change in abundance through six weeks postnatal. At E16, C1qB mRNA was present at high abundance in putative microglia/macrophages in cortical marginal and intermediate zones, and hippocampal analge.

Description: A DNA sequence encoding the human C1QB (NP_000482.3) precursor (Met 1-Ala 253) was expressed, with a carboxy-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 87298828
• NCBI GeneID: 713
• NCBI Accession #: NP_000482.3
• NCBI GenBank Nucleotide #: NM_000491.4
• UniProt Accession #: P02746; Q5T959; Q96H17
• Molecular Weight: 26,722 Da
• NCBI Official Full Name: complement C1q subcomponent subunit B
• NCBI Official Synonym Full Names: complement C1q B chain
• NCBI Official Symbol: C1QB
• NCBI Protein Information: complement C1q subcomponent subunit B
• UniProt Protein Name: Complement C1q subcomponent subunit B
• UniProt Gene Name: C1QB

NCBI Description

This gene encodes the B-chain polypeptide of serum complement subcomponent C1q, which associates with C1r and C1s to yield the first component of the serum complement system. C1q is composed of 18 polypeptide chains which include 6 A-chains, 6 B-chains, and 6 C-chains. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. C1q deficiency is associated with lupus erythematosus and glomerulonephritis. [provided by RefSeq, Dec 2016]

Uniprot Description

C1QB: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. Defects in C1QB are a cause of complement component C1q deficiency (C1QD). A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: complement component C1 complex; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: complement activation; complement activation, classical pathway

Disease: C1q Deficiency