BRMS1 / Breast Cancer Metastasis-Suppressor 1 Recombinant Protein | BRMS1 recombinant protein

Human BRMS1 / Breast Cancer Metastasis-Suppressor 1 Recombinant Protein

• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: BRMS1 / Breast Cancer Metastasis-Suppressor 1; Human BRMS1 / Breast Cancer Metastasis-Suppressor 1 Recombinant Protein; Breast cancer metastasis-suppressor 1; BRMS1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 246

• Applicable Applications for BRMS1 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Homohexamer (Potential). Interacts with SNX6, HDAC1 and RELA. Interacts with ARID4A. Identified in mSin3A corepressor complexes together with SIN3A, SIN3B, RBBP4, RBBP7, SAP30, SUDS3, ARID4A, HDAC1 and HDAC2. Interacts with SPOP; this recruits the protein to a ubiquitin ligase complex containing SPOP and CUL3.
• Entry Function: Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 17530785
• NCBI GeneID: 25855
• NCBI Accession #: NP_056214.1
• NCBI GenBank Nucleotide #: NP_056214.1
• UniProt Accession #: Q9HCU9
• NCBI Official Full Name: breast cancer metastasis-suppressor 1 isoform 1
• NCBI Official Synonym Full Names: BRMS1 transcriptional repressor and anoikis regulator
• NCBI Official Symbol: BRMS1
• NCBI Protein Information: breast cancer metastasis-suppressor 1
• UniProt Protein Name: Breast cancer metastasis-suppressor 1
• Protein Family: Breast cancer metastasis-suppressor
• UniProt Gene Name: BRMS1
• UniProt Entry Name: BRMS1_HUMAN

NCBI Description

This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

BRMS1: Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down- regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma. Belongs to the BRMS1 family.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: NF-kappaB binding; protein binding; histone deacetylase binding; histone deacetylase activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; inhibition of NF-kappaB transcription factor; apoptosis; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent