Mouse Breast cancer metastasis-suppressor 1 (BRMS1) ELISA Kit | BRMS1 elisa kit

Mouse Breast cancer metastasis-suppressor 1 (BRMS1) ELISA Kit

Cat. Num. AAA7249846

• Reactivity: Mouse
• Synonyms: Breast cancer metastasis-suppressor 1 (BRMS1); Mouse Breast cancer metastasis-suppressor 1 (BRMS1) ELISA Kit; BRMS1 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This BRMS1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BRMS1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BRMS1 elisa kit

Priciple of the assay: BRMS1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BRMS1 antibody and an BRMS1-HRP conjugate. The assay sample and buffer are incubated together with BRMS1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BRMS1 concentration since BRMS1 from samples and BRMS1-HRP conjugate compete for the anti-BRMS1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BRMS1 from the sample, fewer sites are left to bind BRMS1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BRMS1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BRMS1 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 17530785
• NCBI GeneID: 25855
• NCBI Accession #: NP_056214.1
• NCBI GenBank Nucleotide #: NM_015399.3
• Molecular Weight: 28,461 Da
• NCBI Official Full Name: breast cancer metastasis-suppressor 1 isoform 1
• NCBI Official Synonym Full Names: breast cancer metastasis suppressor 1
• NCBI Official Symbol: BRMS1
• NCBI Protein Information: breast cancer metastasis-suppressor 1
• UniProt Protein Name: Breast cancer metastasis-suppressor 1
• Protein Family: Breast cancer metastasis-suppressor
• UniProt Gene Name: BRMS1
• UniProt Entry Name: BRMS1_HUMAN

NCBI Description

This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

BRMS1: Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down- regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma. Belongs to the BRMS1 family.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: NF-kappaB binding; protein binding; histone deacetylase binding; histone deacetylase activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; apoptosis; inhibition of NF-kappaB transcription factor; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent